A complete second gut induced by transplanted micromeres in the sea urchin embryo.
A complete second gut induced by transplanted micromeres in the sea urchin embryo.
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海胆胚胎中移植的微粒诱导出完整的第二条肠道。
DOI：
10.1126/science.8438164
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Davidson,EH
中科院分区：
文献类型：
--
作者：
Ransick,A;Davidson,EH
海胆胚胎大多数早期谱系的创始细胞可能是通过诱导性细胞间相互作用来指定的。它示出在这里，一个完整的respecification的细胞命运发生时，16细胞阶段micromeres从植物极的供体胚胎植入到动物极的一个完整的受体胚胎。动物极细胞邻近移植的微粒被重新指定从推定的外胚层到植物板创始人细胞。这些诱导的植物板细胞表达内源性植物板细胞特征性基因的整个电池。异位的植物板内陷原肠胚形成第二原肠分化成一个三方肠，如所示的内胚层特异性标记基因的表达的空间模式。因此，移植的微粒可以向邻近细胞发出信号，诱导它们改变命运。
Founder cells for most early lineages of the sea urchin embryo are probably specified through inductive intercellular interactions. It is shown here that a complete respecification of cell fate occurs when 16-cell stage micromeres from the vegetal pole of a donor embryo are implanted into the animal pole of an intact recipient embryo. Animal pole cells adjacent to the transplanted micromeres are respecified from presumptive ectoderm into vegetal plate founder cells. These induced vegetal plate cells express the entire battery of genes characteristic of the endogenous vegetal plate cells. The ectopic vegetal plate invaginates during gastrulation to form a second archenteron which differentiates properly into a tripartite gut, as shown by the spatial pattern of expression of an endoderm-specific marker gene. Thus, transplanted micromeres can signal neighboring cells to induce them to change their fate.