Expanding the roles of chromatin insulators in nuclear architecture, chromatin organization and genome function.
Expanding the roles of chromatin insulators in nuclear architecture, chromatin organization and genome function.
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扩大染色质绝缘体在核结构、染色质组织和基因组功能中的作用。
DOI：
10.1007/s00018-014-1672-6
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Labrador,Mariano
中科院分区：
文献类型：
--
作者：
Schoborg,Todd;Labrador,Mariano
在参与调节真核细胞染色体结构和功能的众多种类的元件中，染色质绝缘体在体内对这些过程的贡献可以说是最不清楚的。事实上，自从大约四分之一个世纪前，由于最近的全基因组高通量方法更适合于探测这些元件在其天然基因组背景中的作用，我们对染色质绝缘体的看法已经发生了巨大的变化。从这些研究中出现的总体主题是，染色质绝缘体作为高阶染色质环结构的一般促进剂，对基因组施加物理和功能约束。在这篇综述中，我们总结了最近的工作，支持这一想法，以及一些其他的研究将这些元素连接到一个不同的阵列的核过程，表明染色质绝缘体发挥主控制基因组的组织和行为的结果。
Of the numerous classes of elements involved in modulating eukaryotic chromosome structure and function, chromatin insulators arguably remain the most poorly understood in their contribution to these processes in vivo. Indeed, our view of chromatin insulators has evolved dramatically since their chromatin boundary and enhancer blocking properties were elucidated roughly a quarter of a century ago as a result of recent genome-wide, high-throughput methods better suited to probing the role of these elements in their native genomic contexts. The overall theme that has emerged from these studies is that chromatin insulators function as general facilitators of higher-order chromatin loop structures that exert both physical and functional constraints on the genome. In this review, we summarize the result of recent work that supports this idea as well as a number of other studies linking these elements to a diverse array of nuclear processes, suggesting that chromatin insulators exert master control over genome organization and behavior.