Self-cascade reaction catalyzed by CuO nanoparticle-based dual-functional enzyme mimics
Self-cascade reaction catalyzed by CuO nanoparticle-based dual-functional enzyme mimics
复制标题
基于 CuO 纳米粒子的双功能酶模拟物催化的自级联反应
DOI：
10.1016/j.bios.2017.05.037
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发表时间：
2017-11-15
影响因子：
12.6
通讯作者：
Hong, Guo-Lin
中科院分区：
文献类型：
--
作者：
Hu, Ai-Ling;Deng, Hao-Hua;Hong, Guo-Lin
将各种仿生性能组装成一个功能催化级联体系是理想的，但也是具有挑战性的。在这项工作中，研究了纳米氧化铜作为模拟氧化剂，在过氧化氢的作用下，将半胱氨酸氧化为半胱氨酸。将这一特性与CuO纳米粒子的类过氧化物酶活性相结合，我们构建了一个基于单组分纳米酶的自组织级联反应体系，该体系包括半胱氨酸的氧化生成半胱氨酸和过氧化氢，以及过氧化氢介导的对苯二甲酸的氧化产生荧光变化。基于该人工酶促反应体系，建立了半胱氨酸的荧光分析方法，检测下限为6.6 nM。将该平台应用于药品和人体血浆中半胱氨酸的检测，结果令人满意。我们的研究为多功能纳米材料作为酶模拟物的开发和应用开辟了一条新的途径。
It is desirable but challenging to assemble various biomimetic properties into a functional catalytic cascade system. In this work, cupric oxide nanoparticles were investigated as oxidase mimics for the aerobic oxidation of cysteine to cystine with the generation of hydrogen peroxide. Coupling this property with the peroxidase-like activity of CuO nanoparticles, we constructed a self-organized cascade reaction system based on a single-component nanozyme, which includes the oxidation of cysteine to yield cystine and hydrogen peroxide and the hydrogen peroxide-mediated oxidation of terephthalic acid to produce a fluorescence change. Based on this artificial enzymatic cascade reaction system, a fluorometric assay method with a low detection limit of 6.6 nM was established for cysteine determination. This platform was then applied for the detection of cysteine in pharmaceutical products and human plasma, which yielded satisfactory results. Our investigations open up a new route and holds promise for the development and applications of multifunctional nanomaterials as enzyme mimics.