A Suite of Receptor-Like Kinases and a Putative Mechano-Sensitive Channel Are Involved in Autoimmunity and Plasma Membrane-Based Defenses in Arabidopsis.
A Suite of Receptor-Like Kinases and a Putative Mechano-Sensitive Channel Are Involved in Autoimmunity and Plasma Membrane-Based Defenses in Arabidopsis.
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DOI：
10.1094/mpmi-09-16-0184-r
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发表时间：
2017-03
期刊：
影响因子：
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通讯作者：
Zhongqi Zhang;C. Tateda;Shang-Chuan Jiang;J. Shrestha;J. Jeleńska;Dequantarius J. Speed;J. Greenberg
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文献类型：
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作者：
Zhongqi Zhang;C. Tateda;Shang-Chuan Jiang;J. Shrestha;J. Jeleńska;Dequantarius J. Speed;J. Greenberg
在植物中，细胞表面模式识别受体（PRRs）提供了抵御病原体的第一道防线。虽然每个PRR识别特定的配体，但它们共享共同的信号输出，例如胼胝质和其他基于细胞壁的防御。几个PRR对响应于防御信号水杨酸（SA）的愈伤组织诱导也是重要的。PRR信令输出需要公共组件的程度是未知的。功能获得性拟南芥突变体ACCELERATED CELL DEATH 6（ACD 6）acd 6 -1显示部分依赖于PRR的组成型胼胝质产生。ACD 6 -1（和ACD 6）与PRR鞭毛蛋白传感2形成复合物，并且ACD 6是对几种PRR配体的应答所必需的。因此，ACD 6 -1可以作为探针来鉴定对PRR介导的信号传导重要的其他蛋白质。候选信号蛋白（CSP），确定在我们的蛋白质组筛选后，免疫沉淀的血凝素（HA）标记的ACD 6 -1，包括几个亚家族的受体样激酶（RLK）蛋白和机械敏感通道的小传导样4（MSL 4）。在acd 6 -1中，CSP有助于自身免疫。在野生型中，需要CSP来防御细菌和对两种或更多种微生物来源的模式和SA激动剂的胼胝质应答。CSP可用于i）促进信号传导复合物的组装，ii）调节已知PRR的输出，或两者。
In plants, cell surface pattern recognition receptors (PRRs) provide a first line of defense against pathogens. Although each PRR recognizes a specific ligand, they share common signaling outputs, such as callose and other cell wall-based defenses. Several PRRs are also important for callose induction in response to the defense signal salicylic acid (SA). The extent to which common components are needed for PRR signaling outputs is not known. The gain-of-function Arabidopsis mutant of ACCELERATED CELL DEATH6 (ACD6) acd6-1 shows constitutive callose production that partially depends on PRRs. ACD6-1 (and ACD6) forms complexes with the PRR FLAGELLIN SENSING2, and ACD6 is needed for responses to several PRR ligands. Thus, ACD6-1 could serve as a probe to identify additional proteins important for PRR-mediated signaling. Candidate signaling proteins (CSPs), identified in our proteomic screen after immunoprecipitation of hemagglutinin (HA)-tagged ACD6-1, include several subfamilies of receptor-like kinase (RLK) proteins and a MECHANO-SENSITIVE CHANNEL OF SMALL CONDUCTANCE-LIKE 4 (MSL4). In acd6-1, CSPs contribute to autoimmunity. In wild type, CSPs are needed for defense against bacteria and callose responses to two or more microbial-derived patterns and an SA agonist. CSPs may function to either i) promote the assembly of signaling complexes, ii) regulate the output of known PRRs, or both.