Circadian clock genes oscillate in human peripheral blood mononuclear cells
Circadian clock genes oscillate in human peripheral blood mononuclear cells
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DOI：
10.1182/blood-2003-03-0779
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发表时间：
2003-12-01
期刊：
影响因子：
20.3
通讯作者：
Sun, ZS
中科院分区：
文献类型：
--
作者：
Boivin, DB;James, FO;Sun, ZS
在哺乳动物中，有充分的证据表明，可观察到的昼夜节律是由一个中央振荡器控制的，该振荡器组织在涉及几个时钟基因的转录和翻译反馈环中。虽然最近的研究表明时钟基因在许多外周组织中振荡，但它们在人类免疫系统中的特征仍不清楚。本研究旨在探讨生物钟基因在人外周血单核细胞中的功能。根据3名受试者在受控条件下的研究，生物钟基因hPer1、hPer2、hPer3和hDec1在人外周血单个核细胞(PBMCs)中以昼夜节律的方式表达，峰值出现在习惯性活动时间。人外周血单核细胞功能节律机制的证明表明，外周血细胞可能有助于研究人类的昼夜节律及其相关疾病。(C)2003年，由美国血液病学会提供。
In mammals, it is well documented that observable circadian rhythms are controlled by a central oscillator that is organized in transcriptional and translational feedback loops involving several clock genes. Although recent studies have demonstrated that clock genes oscillate in many peripheral tissues, their characteristics in the human immune system remain unknown. The present study investigates whether circadian clock genes function in human peripheral blood mononuclear cells. On the basis of studies derived from 3 human subjects under controlled conditions, circadian clock genes hPer1, hPer2, hPer3, and hDec1 are expressed in a circadian manner in human peripheral blood mononuclear cells (PBMCs), with the peak level occurring during the habitual time of activity. The demonstration of functional circadian machinery in human PBMCs suggests that peripheral blood cells may be useful for the investigation of human circadian rhythms and their associated disorders. (C) 2003 by The American Society of Hematology.