Transposon-driven transcription is a conserved feature of vertebrate spermatogenesis and transcript evolution.
Transposon-driven transcription is a conserved feature of vertebrate spermatogenesis and transcript evolution.
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DOI：
10.15252/embr.201744059
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发表时间：
2017-07
期刊：
影响因子：
7.7
通讯作者：
Enright AJ
中科院分区：
文献类型：
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作者：
Davis MP;Carrieri C;Saini HK;van Dongen S;Leonardi T;Bussotti G;Monahan JM;Auchynnikava T;Bitetti A;Rappsilber J;Allshire RC;Shkumatava A;O'Carroll D;Enright AJ
精子发生与染色体和染色质的重大而独特的变化有关。在这里，我们试图了解这些变化对生精转录的影响。我们发现，特定的小鼠内源性逆转录病毒(ERV)的长末端重复序列(LTrs)驱动了许多长非编码转录本(LncRNA)的表达。这个过程发生在有丝分裂后，主要发生在精母细胞和圆形精子细胞中。我们证明这种转座子驱动的lncRNA表达是脊椎动物精子发生的一个保守特征。我们认为转座子启动子是一种机制，通过它基因组可以探索新的转录底物，增加进化的可塑性，并允许新的编码和非编码基因的发生。因此，我们表明，这些新的ERV驱动的转录本中的一小部分编码短的开放阅读框架，产生可检测到的多肽。最后，我们发现来自相同亚家族的不同ERV元件在组织特异性背景下作为不同激活的启动子。综上所述，我们证明LTRs可以作为组织特异性启动子，并有助于有丝分裂后生精转录体的多样性。
Spermatogenesis is associated with major and unique changes to chromosomes and chromatin. Here, we sought to understand the impact of these changes on spermatogenic transcriptomes. We show that long terminal repeats (LTRs) of specific mouse endogenous retroviruses (ERVs) drive the expression of many long non‐coding transcripts (lncRNA). This process occurs post‐mitotically predominantly in spermatocytes and round spermatids. We demonstrate that this transposon‐driven lncRNA expression is a conserved feature of vertebrate spermatogenesis. We propose that transposon promoters are a mechanism by which the genome can explore novel transcriptional substrates, increasing evolutionary plasticity and allowing for the genesis of novel coding and non‐coding genes. Accordingly, we show that a small fraction of these novel ERV‐driven transcripts encode short open reading frames that produce detectable peptides. Finally, we find that distinct ERV elements from the same subfamilies act as differentially activated promoters in a tissue‐specific context. In summary, we demonstrate that LTRs can act as tissue‐specific promoters and contribute to post‐mitotic spermatogenic transcriptome diversity.