Evaluation of autologous bone marrow-derived nucleated cells for healing of full-thickness skin wounds in rabbits
Evaluation of autologous bone marrow-derived nucleated cells for healing of full-thickness skin wounds in rabbits
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DOI：
10.1111/j.1742-481x.2010.00683.x
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发表时间：
2010-08-01
影响因子：
3.1
通讯作者：
Kumar, Dinesh
中科院分区：
文献类型：
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作者：
Borena, B. M.;Pawde, A. M.;Kumar, Dinesh
本研究的目的是评估自体骨髓源性有核细胞提高兔全层切除皮肤伤口愈合率的潜力。本研究在20只新西兰白色家兔（雌雄不限）中进行。创建两个2 x 2 cm全层皮肤（胸腰区）切除伤口;每只动物背中线两侧各一个。伤口被随机分配到自体骨髓源性有核细胞注射到伤口边缘（BI），或局部应用无菌生理盐水溶液（生理盐水，NS），作为对照。通过评价肉芽组织形成、伤口收缩、上皮形成和组织病理学和组织化学变化来评估伤口愈合，直至伤口形成后28天。BI治疗伤口（3.22 +/- 0.22天）的肉芽组织形成明显快于NS治疗伤口（4.56 +/- 0.47天）。在组织学上观察到BI伤口比NS治疗伤口的上皮形成更好。术后21天，BI伤口的伤口收缩明显大于NS伤口。愈合组织的组织学检查显示，BI伤口中的炎症反应较对照伤口中的炎症反应早期消失、显著更多的新生血管形成、更多的纤维组织增生和胶原蛋白的早期沉积和组织学成熟。结论是，与生理盐水相比，在伤口边缘注射自体骨髓源性有核细胞可诱导家兔切除皮肤伤口愈合更快，质量更好。自体骨髓来源的有核细胞的注射可用于促进兔的大的全层皮肤伤口的愈合。
The aim of the study was to evaluate the potential of autologous bone marrow-derived nucleated cells to enhance the rate of healing of full-thickness excisional skin wounds in rabbits. The study was conducted on 20 New Zealand white rabbits of either sex. Two, 2 x 2 cm full-thickness skin (thoracolumabar region) excisional wounds were created; one on each side of the dorsal midline in each animal. The wounds were randomly assigned to either injection of autologous bone marrow-derived nucleated cells into the wound margins (BI), or topical application of sterile saline solution (normal saline, NS), which served as control. The wound healing was assessed by evaluation of granulation tissue formation, wound contraction, epithelisation and histopathological and histochemical changes up to 28 days after creation of the wound. Granulation tissue appeared significantly faster in BI-treated wounds (3.22 +/- 0.22 days) than in NS-treated wounds (4.56 +/- 0.47 days). Better epithelisation was seen histologically in BI wounds than in NS-treated wounds. Wound contraction was significantly more in BI wounds when compared with NS wounds on 21 post-surgery. Histopathological examination of the healing tissue showed early disappearance of inflammatory reaction, significantly more neovascularisation, and more fibroplasias and early lay down and histological maturation of collagen in BI wounds than in control wounds. It was concluded that injection of autologous bone marrow-derived nucleated cells in the wound margins induced faster and better quality healing of excisional skin wounds in rabbits when compared with normal saline. The injection of autologous bone marrow-derived nucleated cells can be used to promote healing of large full-thickness skin wounds in rabbits.