CONTROL OF LEU OPERON EXPRESSION IN ESCHERICHIA-COLI BY A TRANSCRIPTION ATTENUATION MECHANISM
CONTROL OF LEU OPERON EXPRESSION IN ESCHERICHIA-COLI BY A TRANSCRIPTION ATTENUATION MECHANISM
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DOI：
10.1016/0022-2836(81)90348-x
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发表时间：
1981-01-01
影响因子：
5.6
通讯作者：
CALVO, JM
中科院分区：
文献类型：
--
作者：
WESSLER, SR;CALVO, JM
测定了大肠杆菌leu操纵子1号结构基因leuA前后306个碱基对的DNA序列。该序列的一个显著特征是转录终止信号。在体外，转录确实在这个位点终止，产生大约165个核苷酸的先导RNA。这个先导RNA可能被翻译成含有28个氨基酸的肽，其中4个是相邻的亮氨酸残基。几个碱基互补区域存在于先导RNA中。当比较大肠杆菌和鼠伤寒沙门菌leu leader的核苷酸序列时，这些片段是保守的，而其他片段则有明显的差异。高度保守的区域被认为在leu操纵子表达的衰减控制中起功能作用。
The DNA sequence of 306 base pairs preceding and within the 1st structural gene of the leu operon of E. coli, leuA, was determined. A prominent feature of this sequence is a signal for transcription termination. In vitro, transcription does terminate at this site, yielding a leader RNA of about 165 nucleotides. This leader RNA is potentially translatable into a peptide containing 28 amino acids, 4 of which are adjacent Leu residues. Several regions of base complementarity exist within the leader RNA. When the nucleotide sequences of the leu leaders of E. coli and Salmonella typhimurium are compared, these segments are conserved while other segments vary appreciably. The highly conserved regions are those thought to play a functional role in attenuation control of leu operon expression.