AUTOCATALYTIC SYNTHESIS OF A VIRAL RNA IN VITRO
AUTOCATALYTIC SYNTHESIS OF A VIRAL RNA IN VITRO
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DOI：
10.1126/science.150.3698.884
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发表时间：
1965-01-01
期刊：
影响因子：
56.9
通讯作者：
SPIEGELMAN, S
中科院分区：
文献类型：
--
作者：
HARUNA, I;SPIEGELMAN, S
用从感染RNA噬菌体（Qβ）的大肠杆菌中纯化的RNA依赖性RNA聚合酶（“复制酶”）进行的实验表明，该酶产生与病毒RNA分子量相同的多核苷酸;“复制酶”不能区分多核苷酸与其自身的RNA基因组。通过在低于模板与酶的饱和水平的输入比下开始反应，观察到RNA增加的自催化动力学。这些数据与完整病毒基因组的自我繁殖发生在这个简单系统中的结论一致。
Experiments with an RNA-dependent RNA polymerase ("replicase") purified fromEscherichia coliinfected with an RNA bacteriophage (Qβ) demonstrate that the enzyme generates a polynucleotide of the same molecular weight as viral RNA; the "replicase" cannot distinguish the polynucleotide from its own RNA genome. By starting reactions at input ratios below the saturation levels of template to enzyme, autocatalytic kinetics of RNA increase are observed. The data are consistent with the conclusion that self-propagation of complete viral genomes is occurring in this simple system.