NMDA receptor subunit NR1-immunoreactivity in the rat pons and brainstem and colocalization with Fos induced by nasal stimulation
NMDA receptor subunit NR1-immunoreactivity in the rat pons and brainstem and colocalization with Fos induced by nasal stimulation
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DOI：
10.1016/s0006-8993(98)00885-3
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发表时间：
1998-11-02
期刊：
影响因子：
2.9
通讯作者：
Herbert, H
中科院分区：
文献类型：
--
作者：
Dutschmann, M;Guthmann, A;Herbert, H
在本研究中，我们研究了神经元的分布在臂旁核（PB），Kolliker-Escherichnucleus（KF），三叉神经脊束核尾侧（Sp 5C），孤束核（NTS）和延髓腹外侧区（VLM），这是通过唤起鼻三叉神经反射激活，并表现出N-甲基-D-天冬氨酸（NMDA）受体亚单位NR 1的免疫反应。用生理盐水刺激鼻粘膜，诱导即刻早期基因c-fos的表达，并将Fos蛋白的免疫细胞化学检测与NR 1亚基的检测相结合。细胞计数显示，鼻刺激，麻醉控制相比，导致高度显着增加（p小于或等于0.001）的Fos免疫反应（-ir）神经元的中层KF，外侧PB，和Sp 5C。在中央外侧PB、头端腹外侧延髓（包括Botzinger/pre-Botzinger复合体）以及腹外侧和连合NTS中，增加仅具有中度显著性（p小于或等于0.05）。相对于NR 1-/Fos-ir双标记神经元的数量，仅在这些脑桥延髓核的一个子集中观察到显着增加。Sp 5C（p小于或等于0.001）和中层KF（p小于或等于0.01）的增加非常显著，外侧PB、Botzinger/pre-Botzinger复合体和腹外侧NTS的增加中度显著（p小于或等于0.05）。目前的研究表明，鼻三叉神经激活神经元的强制性和潜在的中继网站的鼻三叉神经反射回路表达NR 1亚单位。这一发现强烈表明，NMDA型谷氨酸受体参与调解的nasotrigeminally诱发的心血管和呼吸反应。(C)1998 Elsevier Science B. V.保留所有权利。
In the present study, we examined the distribution of neurons in the parabrachial nucleus (PB), the Kolliker-Fuse nucleus (KF), the spinal trigeminal nucleus caudalis (Sp5C), the nucleus of the solitary tract (NTS) and the ventrolateral medulla (VLM), which are activated by evoking the nasotrigeminal reflex and which exhibit immunoreactivity for the N-methyl-D-aspartate (NMDA) receptor subunit NR1. By stimulating the nasal mucosa with saline, we induced the expression of the immediate early gene c-fos and combined the immunocytochemical detection of the Fos protein with the detection of the NR1 subunit. Cell counts revealed that nasal stimulation, compared to anesthesia controls, resulted in highly significant increases (p less than or equal to 0.001) of Fos-immunoreactive (-ir) neurons in the midlevel KF, the external lateral PB, and the Sp5C. In the central lateral PB, the rostral ventrolateral medulla including the Botzinger/pre-Botzinger complex, and in the ventrolateral and commissural NTS the increases were only moderately significant(p less than or equal to 0.05). With respect to the numbers of NR1-/Fos-ir double-labeled neurons, significant increases were only observed in a subset of these pontomedullary nuclei. Increases were highly significant in the Sp5C (p less than or equal to 0.001) and the midlevel KF(p less than or equal to 0.01) and moderately significant (p less than or equal to 0.05) in the external lateral PB, Botzinger/pre-Botzinger complex, and ventrolateral NTS. The present study revealed that nasotrigeminally activated neurons in mandatory and potential relay sites of the nasotrigeminal reflex circuit express the NR1 subunit. This finding strongly suggests that NMDA-type glutamate receptors are involved in the mediation of the nasotrigeminally evoked cardiovascular and respiratory responses. (C) 1998 Elsevier Science B.V. All rights reserved.