Selective uptake of intraluminal dextran sulfate sodium and senna by macrophages in the cecal mucosa of the guinea pig
Selective uptake of intraluminal dextran sulfate sodium and senna by macrophages in the cecal mucosa of the guinea pig
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DOI：
10.1007/bf02389517
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发表时间：
1996-04-01
影响因子：
6.3
通讯作者：
Fujino, MA
中科院分区：
文献类型：
--
作者：
Hoshi, O;Iwanaga, T;Fujino, MA
在豚鼠大肠中检测了巨噬细胞参与腔内物质进入固有层的过程。实验中分别引起溃疡性结肠炎和大肠黑素病的右旋糖酐硫酸钠（DSS）和番泻叶被选为研究对象，因为这些物质在显微镜下是可见的，无需任何特殊处理。给豚鼠口服DSS （m500000）和番泻草。在肠组织切片中，甲苯胺蓝染色显示DSS的存在，光镜下可见番泻叶为棕色色素。在豚鼠的大肠中，巨噬细胞在盲肠中数量最多，向直肠方向减少。DSS引起的异色反应首先在盲肠上皮中被发现，随后被巨噬细胞纳入。DSS的存在，无论是上皮细胞还是巨噬细胞，在小肠或远端结肠中都没有被识别出来。番泻色素沉着也局限于盲肠和结肠近端，其中色素巨噬细胞聚集在固有层。这两种不同的口服药物在盲肠中被吸收，部分也在近端结肠中被吸收；这些物质通过上皮并被巨噬细胞吸收，这一发现表明肠道屏障在这一特定部分存在薄弱环节。
The involvement of macrophages in the passage of intraluminal substances into the lamina propria was examined in the large intestine of the guinea pig. Dextran sulfate sodium (DSS) and senna, which, experimentally, induce ulcerative colitis and melanosis coli, respectively, were chosen for examination, since these substances are visible under the microscope without any special treatment. DSS (MW 50000) and senna were orally administered to guinea pigs. In tissue sections of the intestine, the presence of DSS was demonstrated by toluidine blue staining, while senna was visible under the light microscope as brown pigment. In the large intestine of guinea pigs, macrophages were most numerous in the cecum, decreasing in number towards the rectum. Metachromatic reaction due to DSS was first recognized in the epithelium of the cecum, and was subsequently incorporated by macrophages. The presence of DSS, either in the epithelium or in macrophages, was not recognized in the small intestine or the distal colon. Senna pigmentation was also limited to the cecum and proximal colon, in which pigmented macrophages aggregated in the lamina propria. The two different substances administered orally were taken up in the cecum, and partly also in the proximal colon; the substances passed through the epithelium and were incorporated by macrophages, This finding suggests the existence of a weak point in the intestinal barrier in this particular portion of the intestine.