The Endoplasmic Reticulum Provides the Membrane Platform for Biogenesis of the Flavivirus Replication Complex
The Endoplasmic Reticulum Provides the Membrane Platform for Biogenesis of the Flavivirus Replication Complex
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DOI：
10.1128/jvi.00986-10
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发表时间：
2010-10-01
影响因子：
5.4
通讯作者：
Mackenzie, Jason M.
中科院分区：
文献类型：
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作者：
Gillespie, Leah K.;Hoenen, Antje;Mackenzie, Jason M.
正义RNA病毒的细胞质复制与宿主细胞膜的剧烈重排有关。这些病毒诱导的变化导致包裹病毒复制复合物（RC）的囊泡结构的诱导。在这项研究中，我们扩展了之前对西尼罗河病毒株昆金病毒（WNVKUN）细胞内位置的观察，表明病毒诱导的宿主蛋白和膜的募集似乎发生在高尔基体前的步骤。为了可视化 WNVKUN 复制复合体，我们对 WNVKUN 复制子转染细胞的断层图进行了三维 (3D) 建模。这些分析提供了复制复合物的 3D 表示，揭示了复制复合物与细胞质的开放通道以及复合物与粗面内质网的流动性。此外，我们提供的数据表明，RC 内的大多数病毒 RNA 都是双链 RNA (dsRNA) 形式。
The cytoplasmic replication of positive-sense RNA viruses is associated with a dramatic rearrangement of host cellular membranes. These virus-induced changes result in the induction of vesicular structures that envelop the virus replication complex (RC). In this study, we have extended our previous observations on the intracellular location of West Nile virus strain Kunjin virus (WNVKUN) to show that the virus-induced recruitment of host proteins and membrane appears to occur at a pre-Golgi step. To visualize the WNVKUN replication complex, we performed three-dimensional (3D) modeling on tomograms from WNVKUN replicon-transfected cells. These analyses have provided a 3D representation of the replication complex, revealing the open access of the replication complex with the cytoplasm and the fluidity of the complex to the rough endoplasmic reticulum. In addition, we provide data that indicate that a majority of the viral RNA species housed within the RC is in a double-stranded RNA (dsRNA) form.