Atmospheric pressure laser desorption/ionization on porous silicon.
Atmospheric pressure laser desorption/ionization on porous silicon.
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DOI：
10.1002/rcm.781
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发表时间：
2002-09
期刊：
影响因子：
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通讯作者：
Victor V. Laiko;N. Taranenko;V. Berkout;B. Musselman;V. Doroshenko
中科院分区：
文献类型：
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作者：
Victor V. Laiko;N. Taranenko;V. Berkout;B. Musselman;V. Doroshenko
最近开发的商业大气压基质辅助激光解吸/电离（AP-MALDI）源（MassTech，Inc.）修改为采用市售的DIOS板（Mass Consortium Corp.）用于研究多孔硅表面在大气压条件下的激光解吸。研究了多孔硅表面大气压激光解吸电离（AP-DIOS）的可行性。这种新的AP-DIOS技术的优点包括相当好的灵敏度（标准肽混合物的亚皮摩尔范围），样品制备简单，目标斑点的均匀性和光谱中没有基质峰。AP-DIOS源与商业离子阱（LCQ Classic，Thermo Finnigan）连接，其另外提供独特的MS（n）能力。将250 fmol未分离的牛血清白蛋白（BSA）胰蛋白酶消化物的AP-DIOS光谱与AP-MALDI光谱进行比较。AP-DIOS对质量范围为200-1000 Da的消化组分提供了更好的覆盖率。与AP-DIOS或AP-MALDI单独分析相比，两种技术的组合数据使我们能够在BSA消化分析中匹配峰的数量几乎增加一倍。
A recently developed commercial atmospheric pressure matrix-assisted laser desorption/ionization (AP-MALDI) source (MassTech, Inc.) was modified to adopt commercially available DIOS plates (Mass Consortium Corp.) for the studies of laser desorption from the surface of porous silicon under atmospheric pressure conditions. The feasibility of atmospheric pressure laser desorption/ionization from the surface of porous silicon (AP-DIOS) was demonstrated. The advantages of this new AP-DIOS technique include reasonably good sensitivity (subpicomole range for standard peptide mixtures), simplicity of sample preparation, uniformity of target spots and the absence of matrix peaks in the spectra. The AP-DIOS source was interfaced with a commercial ion trap (LCQ Classic, Thermo Finnigan) which additionally provides a unique MS(n) capability. The AP-DIOS spectrum of 250 fmol of unseparated tryptic digest of bovine serum albumin (BSA) was compared with that of AP-MALDI for the same compound. AP-DIOS offers significantly better coverage for the digest components in the mass range 200-1000 Da. The combined data of both techniques enabled us to nearly double the number of matched peaks in BSA digest analysis compared with AP-DIOS or AP-MALDI analysis separately.