The rice LysM receptor-like kinase OsCERK1 is required for the perception of short-chain chitin oligomers in arbuscular mycorrhizal signaling
The rice LysM receptor-like kinase OsCERK1 is required for the perception of short-chain chitin oligomers in arbuscular mycorrhizal signaling
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DOI：
10.1111/nph.14539
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发表时间：
2017-06-01
期刊：
影响因子：
9.4
通讯作者：
Genre, Andrea
中科院分区：
文献类型：
--
作者：
Carotenuto, Gennaro;Chabaud, Mireille;Genre, Andrea
水稻赖氨酸基序（LysM）受体样激酶OsCERK 1现在已知在致病和共生相互作用中具有双重作用。在最近发现Oscerk 1突变体不能宿主丛枝菌根（AM）真菌之后，我们已经研究了OsCERK 1是否直接参与AM真菌分泌物中鉴定的短链几丁质寡聚体（Myc-CO）的感知，并显示其激活水稻根表皮中的核钙（Ca 2+）尖峰。用YC 2.60检测了AM真菌分泌物和Myc-COs处理水稻根系后细胞核内Ca 2+的变化，结果表明，与野生型相比，Oscerk 1突变体的根系成纤维细胞对AM真菌诱导的Ca 2+反应不明显。而OsCEBiP突变的水稻株系则对Myc-CO反应阳性，该突变体编码LysM受体样蛋白，与OsCERK 1结合感知宿主免疫防御途径的几丁质激发子，这为双功能OsCERK 1在感知短链Myc-CO信号和激活下游保守的共生信号转导途径中发挥重要作用提供了直接证据。
The rice lysin-motif (LysM) receptor-like kinase OsCERK1 is now known to have a dual role in both pathogenic and symbiotic interactions. Following the recent discovery that the Oscerk1 mutant is unable to host arbuscular mycorrhizal (AM) fungi, we have examined whether OsCERK1 is directly involved in the perception of the short-chain chitin oligomers (Myc-COs) identified in AM fungal exudates and shown to activate nuclear calcium (Ca2+) spiking in the rice root epidermis.An Oscerk1 knockout mutant expressing the cameleon NLS-YC2.60 was used to monitor nuclear Ca2+ signaling following root treatment with either crude fungal exudates or purified Myc-COs.Compared with wild-type rice, Ca2+ spiking responses to AM fungal elicitation were absent in root atrichoblasts of the Oscerk1 mutant. By contrast, rice lines mutated in OsCEBiP, encoding the LysM receptor-like protein which associates with OsCERK1 to perceive chitin elicitors of the host immune defense pathway, responded positively to Myc-COs.These findings provide direct evidence that the bi-functional OsCERK1 plays a central role in perceiving short-chain Myc-CO signals and activating the downstream conserved symbiotic signal transduction pathway.