The Herpes simplex virus type 1 UL6 protein is essential for cleavage and packaging but not for genomic inversion
The Herpes simplex virus type 1 UL6 protein is essential for cleavage and packaging but not for genomic inversion
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DOI：
10.1006/viro.1996.0668
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发表时间：
1996-12-15
期刊：
影响因子：
3.7
通讯作者：
Weller, SK
中科院分区：
文献类型：
--
作者：
Lamberti, C;Weller, SK
HSV-1基因组由两侧为反向重复序列的两个独特区域（U-L和U-S）组成。在DNA复制过程中，两个独特的区域U和U相对于彼此反转。在这份报告中，我们提出了切割不是基因组倒位发生所必需证据。我们分离并鉴定了一种UL 6：：lacZ插入突变体（hr 74），其产生野生型水平的复制病毒DNA，但却切割和包装DNA。我们证明，这种病毒仍然能够进行基因组倒位。此外，我们证实，从野生型病毒感染的细胞中复制的DNA含有特定的U-L末端，但不含U-S末端，而突变型hr 74感染的细胞不含U-S或U-L末端。这表明在复制DNA中发现的特异性U-L末端是切割/包装过程的结果。(C)出版社：Academic Press，Inc.
The HSV-1 genome is composed of two unique regions (U-L and U-S) flanked by inverted repeats. During the course of DNA replication the two unique regions U, and U, invert relative to one another. in this report we present evidence that cleavage is not necessary for genomic inversion to occur. We isolated and characterized a UL6::lacZ insertion mutant (hr74) that produces wild-type levels of replicating viral DNA but rails to cleave and package DNA. We demonstrate that this virus is still able to undergo genomic inversion. Furthermore we confirm that replicating DNA from cells infected with wild-type virus contains specific U-L termini but not U-S termini, whereas cells infected with the mutant hr74 do not contain either U-S or U-L termini. This demonstrates that the specific U-L ends found in replicating DNA are the result of the cleavage/packaging process. (C) 1996 Academic Press, Inc.