Comparative analysis of motility and other properties of Treponema denticola strains
Comparative analysis of motility and other properties of Treponema denticola strains
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DOI：
10.1016/j.micpath.2016.11.021
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发表时间：
2017-01-01
影响因子：
3.8
通讯作者：
Yoshimura, Fuminobu
中科院分区：
文献类型：
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作者：
Nagano, Keiji;Hasegawa, Yoshiaki;Yoshimura, Fuminobu
牙周炎相关病原体密螺旋体是一种螺旋体细菌，它利用质周鞭毛旋转其细胞体，像螺旋钻一样游泳。我们比较了四株齿状霉的生理和致病特性，包括运动性。相衬显微镜显示菌株之间的运动性差异；其中，ATCC 35404活性最高，ATCC 33521次之，其余2株（ATCC 35405和ATCC 33520）活性最低。透射电镜显示，低运动菌株的鞭毛呈细胞外突状，由鞭毛拉长引起。密螺旋体鞭毛丝由FlaB1、FIaB2和FIaB3三种鞭毛蛋白组成。菌株间FIaB1表达量具有可比性，而菌株ATCC 35404中FIaB2表达量最低。FlaB3的表达量在不同菌株间存在差异，ATCC 35405、ATCC 33520、ATCC 33521和ATCC 35404的表达量分别最高和最低。此外，低运动菌株的FIaB3的电泳迁移速度更快，这表明这些蛋白的翻译后修饰可能有所不同，因为菌株之间的FlaB3氨基酸序列相同。这些结果表明，不适当的FIaB2和FIaB3表达导致鞭毛异常伸长，导致运动性下降。此外，与高运动性菌株相比，低运动性菌株的细菌密度更高，乳糜蛋白酶样蛋白酶活性更高，与牙龈上皮细胞相关的细菌细胞更多。运动和这些特性之间可能存在联系，但这种联系背后的遗传因素尚不清楚。(C) 2016 Elsevier Ltd.版权所有。
The periodontitis-associated pathogen Treponema denticola is a spirochetal bacterium that swims by rotating its cell body like a corkscrew using periplasmic flagella. We compared physiologic and pathogenic properties, including motility, in four strains of T. denticola. Phase-contrast microscopy showed differential motility between the strains; ATCC 35404 showed the highest motility, followed by ATCC 33521, and the remaining two strains (ATCC 35405 and ATCC 33520) showed the lowest motility. Transmission electron microscopy showed that the low motility strains exhibited extracellular flagellar protrusions resulting from elongated flagella. Treponemal flagellar filaments are composed of three flagellins of FlaB1, FIaB2 and FIaB3. FIaB1 expression was comparable between the strains, whereas FIaB2 expression was lowest in ATCC 35404. FlaB3 expression varied among strains, with ATCC 35405, ATCC 33520, ATCC 33521, and ATCC 35404 showing the highest to lowest expression levels, respectively. Additionally, the low motility strains showed faster electrophoretic mobility of FIaB3, suggesting that posttranslational modifications of these proteins may have varied, because the amino acid sequences of FlaB3 were identical between the strains. These results suggest that inappropriate expression of FIaB2 and FIaB3 caused the unusual elongation of flagella that resulted in decreased motility. Furthermore, the low motility strains grew to higher bacterial density, and showed greater chymotrypsin-like protease activity, and more bacterial cells associated with gingival epithelial cells in comparison with the high motility strains. There may be a relationship between motility and these properties, but the genetic factors underlying this association remain unclear. (C) 2016 Elsevier Ltd. All rights reserved.