An immunoperoxidase monolayer assay for the detection of antibodies against swine influenza virus
An immunoperoxidase monolayer assay for the detection of antibodies against swine influenza virus
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DOI：
10.1177/104063870201400215
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发表时间：
2002-03-01
影响因子：
1.5
通讯作者：
Goyal, SM
中科院分区：
文献类型：
--
作者：
Direksin, K;Joo, H;Goyal, SM
免疫过氧化物酶单层测定 (IPMA) 已被开发用于检测猪血清中的甲型猪流感病毒 (SIV) 抗体。该测试使用来自实验感染 H1N1 亚型 SIV 的猪的连续血清进行评估。还测试了经过血凝抑制（HI）测试的 200 个现场血清样本。 IPMA 测试中最早在接种后 3 天 (dpi) 就检测到了 SIV 特异性抗体，而 HI 测试则需要 7 dpi。与 HI 不同，IPMA 测试不需要血清处理。无论测试中使用哪种病毒，IPMA 都会检测到 SIV 的 H1N1 和 H3N2 亚型抗体，而 HI 会检测到 H1N1 或 H3N2 抗体，具体取决于测试中使用的病毒。这项研究的结果表明，IPMA 是筛选猪血清中 SIV 抗体的有用测试。
An immunoperoxidase monolayer assay (IPMA) has been developed to detect antibodies against swine influenza A virus (SIV) in pig sera. The test was evaluated by using sequential sera from pigs experimentally infected with H1N1 subtype of SIV. Two hundred field serum samples that had been examined by the hemagglutination inhibition (HI) test were also tested. Antibodies specific to SIV were detected as early as 3 days postinoculation (dpi) in the IPMA test as compared with 7 dpi by the HI test. Unlike HI, no serum treatment was required in the IPMA test. Regardless of the virus used in the test, IPMA detected antibodies to both H1N1 and H3N2 subtypes of SIV whereas HI detects antibodies against either H1N1 or H3N2, depending upon the virus used in the test. Results of this study indicate that IPMA is a useful test for screening of pig sera for SIV antibodies.