Morphogenesis induction and organogenic nodule differentiation in Populus euphratica Oliv. leaf explants
Morphogenesis induction and organogenic nodule differentiation in Populus euphratica Oliv. leaf explants
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DOI：
10.1007/s11240-008-9457-y
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发表时间：
2009-01-01
影响因子：
3
通讯作者：
Pais, Maria Salomé
中科院分区：
文献类型：
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作者：
Ferreira, Silvia;Batista, Dora;Pais, Maria Salomé
胡杨。是一种落叶杨树，主要分布在中国和中东国家的河岸地区，对极端温度和高土壤盐度具有很高的耐受性。本研究报道了一种基于形态发生过程的胡杨叶片外植体发育和繁殖的优化方案，该过程涉及有机根瘤分化。在α -萘乙酸和6-苄基氨基嘌呤的浓度范围内，以固定的2:1的比例，胡杨叶片外植体的有机根瘤可以实现不定芽再生。在培养基上接种后第5天形成层细胞开始分裂，12天后已形成若干组织中心。不易碎的愈伤组织与组织中心形成结构，在40天后演变成结节，然后在50-60天后能够再生新芽。这些根瘤没有从母体外植体中分离出来，并且能够成功地产生新的不定芽。在不同的培养基中成功地培养和生根，获得发育完全的植株。这里描述的胡杨再生系统是创新的，可复制的，形态学研究过程的数据支持再生结构作为有机结节的分类。
Populus euphratica Oliv. is a deciduous poplar species, occurring mainly in riparian areas of China and Middle Eastern countries, and presenting high tolerance to extreme temperatures and high soil salinity. In this study, an optimized protocol for development and propagation of P. euphratica from leaf explants is reported, based on a morphogenic process that involves organogenic nodule differentiation. Adventitious shoot regeneration of P. euphratica from organogenic nodules of leaf explants was achieved within a range of concentrations of alpha-naphtalenacetic acid and 6-benzylaminopurine, at a fixed 2:1 ratio. Cambial cells started to divide 5 days after inoculation on culture medium and, after 12 days, several organizing centres were already formed. Non-friable callus tissue, together with organization centres, formed structures that evolved to nodules after about 40 days which were, then, able to regenerate new shoots after 50-60 days. The nodules did not separate from the mother explants and were able to successfully give rise to new adventitious shoots. These were rescued and successfully grown and rooted in different culture media, and fully developed plants were obtained. The regeneration system here described for P. euphratica is innovative, reproducible and data from histological studies of the morphogenic process support the classification of the regenerative structures as organogenic nodules.