Theoretical investigation of ultrasound-modulated Cerenkov luminescence imaging for higher-resolution imaging in turbid media.
Theoretical investigation of ultrasound-modulated Cerenkov luminescence imaging for higher-resolution imaging in turbid media.
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DOI：
10.1364/ol.43.003509
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发表时间：
2018-08-01
期刊：
影响因子：
3.6
通讯作者：
Cherry SR
中科院分区：
文献类型：
--
作者：
Klein JS;Mitchell GS;Stephens DN;Cherry SR
切伦科夫发光成像(CLI)是一种用于活体成像放射性标记分子的光学技术。它已经在临床和临床前环境中证明了实用性，在某些情况下可以作为核成像仪器的替代品。然而，光学散射从根本上限制了使用这种方式可以实现的成像的分辨率和深度。在这篇手稿中，我们报告了支持超声调制切伦科夫发光成像(USCLI)的潜在的数值结果，USCLI是一种可以减少光学散射的新成像方式。这项技术使用声波保持来调制介质的折射率，从而以空间精确的方式调制切伦科夫发光的强度。这种对比机制以前还没有报道过。对于生理兼容的1 Mpa的超声峰值压力，~0.1%的切伦科夫信号可以被调制。此外，我们的模拟表明，USCLI可以克服CLI分辨率的散射限制，并提供更高的分辨率成像。对于以1厘米~3的模拟组织体模为中心的18F点源，散射系数μS‘=10厘米-1，横向空间分辨率为2 mm半高全宽(FWHM)是可能的，分辨率比用激光成像的相同体模精细3倍。
Cerenkov luminescence imaging (CLI) is an optical technique for imaging radiolabeled molecules in vivo. It has demonstrated utility in both the clinical and preclinical settings and can serve as a substitute for nuclear imaging instrumentation in some cases. However, optical scattering fundamentally limits the resolution and depth of imaging that can be achieved with this modality. In this manuscript, we report numerical results that support the potential for ultrasound-modulated Cerenkov luminescence imaging (USCLI), a new imaging modality that can mitigate optical scattering. The technique uses an acoustic Held to modulate the refractive index of the medium and thus the intensity of Cerenkov luminescence in a spatially precise manner. This mechanism of contrast has not been reported previously. For a physiologically compatible ultrasound peak pressure of 1 MPa, ~0.1% of the Cerenkov signal can be modulated. Furthermore, our simulations show that USCLI can overcome the scattering limit of resolution for CLI and provide higher resolution imaging. For an18F point source centered in a 1 cm3 simulated tissue phantom with a scattering coefficient of μs ‘ = 10 cm-1, <2 mm full width at half max (FWHM) lateral spatial resolution is possible, a resolution 3 times finer than the same phantom imaged with CLI.