Changes in the metabolite profile of breast milk over lactation stages and their relationship with dietary intake in Chinese women: HPLC-QTOFMS based metabolomic analysis
Changes in the metabolite profile of breast milk over lactation stages and their relationship with dietary intake in Chinese women: HPLC-QTOFMS based metabolomic analysis
复制标题
中国女性哺乳期母乳代谢物的变化及其与膳食摄入的关系：基于 HPLC-QTOFMS 的代谢组学分析
DOI：
10.1039/c8fo01005f
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发表时间：
2018-10-01
期刊：
影响因子：
6.1
通讯作者：
Li, Duo
中科院分区：
文献类型：
--
作者：
Li, Kelei;Jiang, Jiajing;Li, Duo
本研究的目的是通过基于HPLC-QTOFMS的代谢组学分析，全面评价中国女性母乳中代谢物在不同哺乳期的变化及其与膳食摄入的关系。采集30例健康哺乳期妇女的初乳、过渡乳和成熟乳进行分析。共鉴定出84种代谢产物，包括12种脂肪酰、15种甘油脂、23种甘油磷脂、7种鞘脂、7种维生素、5种核苷酸相关代谢产物、2种氨基酸、1种氨基酸衍生物、9种二肽、1种甾体激素、1种能量相关代谢产物和1种胺。偏最小二乘回归分析表明，初乳，过渡乳和成熟乳的代谢产物谱与膳食摄入量有很强的相关性（R-2值分别为0.92，0.87和0.74）。然而，在84种不同代谢产物中，只有2种与膳食摄入量有较强的相关性：1，24，25-（OH）（3）维生素D-3与肉、蛋、蛋白质和脂肪的膳食摄入量呈正相关; 11-羟孕酮与水果和碳水化合物的膳食摄入量呈负相关。调整膳食摄入量后，所有84种差异代谢产物在泌乳阶段的变化趋势保持不变。结果表明，中国妇女母乳中存在84种差异代谢物，其变化趋势与膳食摄入无关。这些代谢物与西方人群中先前代谢组学研究中鉴定的代谢物部分不同。本研究对了解我国不同发育阶段婴儿营养需要的变化规律，研制适合我国婴儿的配方奶粉具有重要意义。
The aim of the present study was to comprehensively evaluate the changes in the metabolite profile of breast milk over lactation stages and their relationship with dietary intake in Chinese women by HPLC-QTOFMS based metabolomic analysis. The colostrum, transitional milk and mature milk of thirty healthy lactating women were collected for analysis. Eighty-four differential metabolites over lactation stages were identified, including 12 fatty acyls, 15 glycerolipids, 23 glycerophospholipids, 7 sphingolipids, 7 vitamins, 5 nucleotides-related metabolites, 2 amino acids, 1 amino acid derivate, 9 dipeptides, 1 steroid hormone, 1 energy-related metabolite and 1 amine. Partial least-squares regression analysis indicated that the metabolite profiles of the colostrum, transitional milk and mature milk have a strong relationship with dietary intake (R-2 values were 0.92, 0.87 and 0.74, respectively). However, among the 84 differential metabolites over lactation stages, only two showed a strong relationship with dietary intake: 1,24,25-(OH)(3) vitamin D-3 was positively correlated with the dietary intake of meat and eggs, protein and fat; 11-hydroxyprogesterone was negatively correlated with the dietary intake of fruit and carbohydrate. After adjusting for dietary intake, the variation trend of all the 84 differential metabolites over lactation stages remained unchanged. In conclusion, 84 differential metabolites in the breast milk of Chinese women over lactation stages were identified, and their variation trend was independent of dietary intake. These metabolites were partially different from those identified in previous metabolomic studies in the Western population. The present study is quite meaningful for understanding the variation of nutritional requirements in Chinese infants at different developmental stages and manufacturing optimal infant formulas for them.