Body wall repair using small intestinal submucosa seeded with cells
Body wall repair using small intestinal submucosa seeded with cells
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DOI：
10.1016/j.jpedsurg.2003.08.019
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发表时间：
2003-12-01
影响因子：
2.4
通讯作者：
Lin, JN
中科院分区：
文献类型：
--
作者：
Lai, JY;Chang, PY;Lin, JN
背景/目的：腹侧腹壁大面积缺损的人工修补术与高并发症发生率相关。方法：取同系刘易斯大鼠骨骼肌标本，采用组织工程方法进行体外培养。一旦在体外扩增，将骨骼肌细胞或成纤维细胞悬浮在胶原凝胶中。所有动物均创建2.5- X 3-cm腹壁缺损。将细胞种植凝胶置于两片小肠粘膜下层（SIS）之间修复缺损。对照组采用SIS加脱细胞凝胶修复。在不同时间点处死动物进行组织学和力学检查。结果：腹壁疝分别存在于24个成纤维细胞接种的结构中的6个（25%）、21个骨骼肌细胞接种的结构中的5个（23.9%）和21个脱细胞移植物中的16个（76.2%）（P <0.05）。收获时，细胞接种的结构更厚，更好的细胞浸润，而脱细胞移植物薄，细胞密度低，和差的机械resistance.Conclusions：与脱细胞胶原基质，工程细胞结构有更好的细胞浸润和机械性能。组织工程可能是一个可行的替代体壁。
Background/Purpose: Prosthetic repair of large ventral abdominal wall defects has been associated with high complication rates. This study was aimed at applying tissue engineering to body wall replacement.Methods: Syngeneic Lewis rats underwent harvest of skeletal muscle specimens. Once expanded in vitro, skeletal muscle cells or fibroblasts were suspended in a collagen gel. All animals underwent creation of a 2.5- X 3-cm abdominal wall defect. The defect was repaired with the cell-seeded gel placed in between 2 pieces of small intestinal submucosa (SIS). The control group was repaired by SIS with acellular gel. Animals were killed at different time-points for histologic and mechanical examination. Statistical analysis was by analysis of variance (ANOVA).Results: Abdominal wall hernia was present in 6 of 24 fibroblast-seeded constructs (25%), 5 of 21 skeletal muscle cell-seeded constructs (23.9%), and 16 of 21 acellular grafts (76.2%), respectively (P < .05). At harvest, cell-seeded constructs were thicker with better cellular infiltration, whereas acellular grafts were thin, low in cell density, and poor in mechanical resistance.Conclusions: Unlike acellular collagen matrices, engineered cellular constructs have better cell infiltration and mechanical performance. Tissue engineering may be a viable alternative for body-wall replacement.