Efficacy of low-molecular-weight heparin and unfractionated heparin to prevent adhesion of human prostate and bladder carcinoma and melanoma cells to bovine endothelial monolayers - An in vitro study and review of the literature
Efficacy of low-molecular-weight heparin and unfractionated heparin to prevent adhesion of human prostate and bladder carcinoma and melanoma cells to bovine endothelial monolayers - An in vitro study and review of the literature
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DOI：
10.1159/000282848
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发表时间：
1996-01-01
影响因子：
1.6
通讯作者：
Classen, M
中科院分区：
文献类型：
--
作者：
Lersch, C;Gericke, D;Classen, M
以0.5、1、5、10、20IU的低分子肝素(LMWH)、普通肝素(UFH)和生理盐水为对照，检测了人前列腺癌(PC 3，DU 145)、膀胱癌(647 V，J 82)和黑色素瘤(RPMI-8252)细胞系对塑料和牛内皮细胞单层(BEM)的粘附力。培养液中添加5%胎牛血清或人血浆，分别于培养2、4、2 4h和1.5、3、2 4h在无BEM的微量平板上计数肿瘤细胞。20IU的低分子肝素可增加膀胱癌细胞对BEM的黏附，20IU的超滤过高可增加DU-145前列腺癌和RPMI-8252黑色素瘤细胞的黏附能力。LMWH明显不能比UFH更有效地阻止人前列腺癌、膀胱癌和黑色素瘤细胞与BEM的黏附。UFH和LMWH阻止静脉内肿瘤细胞在血管内定植的体内效应显然不依赖于单独与内皮细胞的相互作用。
Adherence of human prostate carcinoma (PC 3, DU 145), bladder (647 V, J 82) carcinoma and melanoma (RPMI-8252) cell lines to plastic and bovine endothelial monolayers (BEM) was tested in the presence of 0.5, 1, 5, 10, 20 IU of low-molecular-weight heparin (LMWH), unfractionated heparin (UFH) or saline as a control. Culture medium was supplemented either with 5% fetal calf serum or with human plasma, Floating tumor cells were counted after 2, 4, 24 h of culture in microtiter plates without BEM and after 1.5, 3 and 24 h of culture on BEM. Twenty IU of LMWH increased the numbers of bladder carcinoma cells adhering to BEM as did 20 IU of UFH in cultures of DU 145 prostate carcinoma and RPMI-8252 melanoma cells. LMWH obviously does not prevent human prostate and bladder carcinoma and melanoma cells from adhering to BEM more effectively than UFH. The in vivo effect of UFH and perhaps also LMWH that hinders intravenous tumor cell colonization in blood vessels obviously does not depend on the interaction with endothelial cells alone.