Saccharification of ionic-liquid-pretreated sugar cane bagasse using Penicillium echinulatum enzymes
Saccharification of ionic-liquid-pretreated sugar cane bagasse using Penicillium echinulatum enzymes
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DOI：
10.1016/j.jtice.2014.04.017
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发表时间：
2014-09
影响因子：
5.7
通讯作者：
K. Aver;A. Scortegagna;R. C. Fontana;M. Camassola
中科院分区：
文献类型：
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作者：
K. Aver;A. Scortegagna;R. C. Fontana;M. Camassola
基于离子液体(IL)预处理的木质纤维材料溶解是一种很有前途的生物乙醇生产技术。在本研究中，甘蔗渣用乙酸乙酯-3-甲基咪唑([Ac])、1-丁基-3-甲基咪唑醋酸盐([BMIM][Ac])、1-丁基-3-甲基咪唑氯([BMIM][Cl])、1-丁基-3-甲基咪唑溴([BMIM][BR])和1-丁基-3-甲基咪唑甲基硫酸盐([BMIM][CH3SO4])在80°C和120°C两个温度下进行了预处理。用来自棘球青霉的纤维素酶进行水解，并测量还原糖(RS)和葡萄糖的释放量。用扫描电子显微镜(SEM)分析了其结构变化。[BMIM][Cl]和[BMIM][Ac]ILs的RS释放量最高，在80℃和120℃时的浓度分别为83m g/g和219m g/g，分别为471m g/g和728m g/g，经[Emim][Ac]和[BMIM][Ac]预处理后获得了更高的葡萄糖和RS的产率，这与扫描电子显微镜的图像一致，表明在这些情况下生物质表面发生了变化。此外，在120℃和12h的水解条件下，蔗糖的释放速率也有所提高。
The dissolution of lignocellulosic materials based on ionic liquid (IL) pretreatment represents a promising technology for bioethanol production. In this study, sugarcane bagasse was submitted to pretreatment with 1-ethyl-3-methylimidazolium acetate ([EMIM][Ac]), 1-butyl-3-methylimidazolium acetate ([BMIM][Ac]), 1-butyl-3-methylimidazolium chloride ([BMIM][Cl]), 1-butyl-3-methylimidazolium bromide ([BMIM][Br]) and 1-butyl-3-methylimidazolium methylsulphate ([BMIM][CH3SO4]) at two temperatures, 80 °C and 120 °C. To quantify the results, the samples were washed with distilled water, hydrolysed using cellulases fromPenicillium echinulatumand measured in terms of the amounts of reducing sugar (RS) and glucose released. Structural changes were analysed using a scanning electron microscope (SEM). The highest releases of RS were obtained for the [BMIM][Cl] and [BMIM][Ac] ILs; the respective concentrations were 83 mg/g and 219 mg/g at 80 °C and 471 mg/g and 728 mg/g at 120 °C. Higher glucose and RS yields were obtained following pretreatment with [EMIM][Ac] and [BMIM][Ac], in agreement with the SEM images, which showed that the biomass surface actually changed in these cases. Furthermore, the rate of sugar release was increased at temperature of 120 °C and after 12 h of hydrolysis.