Harmine promotes molar root development via SMAD1/5/8 phosphorylation.
Harmine promotes molar root development via SMAD1/5/8 phosphorylation.
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DOI：
10.1016/j.bbrc.2017.12.062
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发表时间：
2017-12
影响因子：
3.1
通讯作者：
N. Fujiwara;Ji-Won Lee;Mika Kumakami-Sakano;K. Otsu;J. Woo;S. Iseki;M. Ota
中科院分区：
文献类型：
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作者：
N. Fujiwara;Ji-Won Lee;Mika Kumakami-Sakano;K. Otsu;J. Woo;S. Iseki;M. Ota
牙齿和骨骼是参与体内生理钙化的主要组织，它们使用相似的分子途径进行发育、体内平衡和再生。毒碱（HMN）是一种天然的小化合物，可刺激体外和体内成骨细胞分化。我们研究了HMN对出生后磨牙根和牙周组织发育的生物学效应。HMN支持牙根和牙周组织在牙胚发育过程中的形成。在牙胚器官培养中，HMN促进了Hertwig’s上皮根鞘（Hertwig’s epithelial root sheath， HERS）的伸长，刺激了Hertwig’s上皮根鞘和牙毛囊来源组织（包括牙乳头和牙毛囊）的细胞增殖。HMN刺激hers源性细胞在体外无间充质细胞的增殖和细胞运动，并直接诱导hers源性细胞SMAD1/5/8蛋白磷酸化。我们的研究结果表明，HMN是第一个天然的小化合物，刺激出生后的牙齿细菌的发展。
Tooth and bone are major tissues involved in physiological calcification in the body, and they use similar molecular pathways for development, homeostasis, and regeneration. Harmine (HMN) is a natural small compound that stimulates osteoblast differentiationin vitroandin vivo. Here we examined the biological effect of HMN on the postnatal development of molar tooth roots and periodontal tissues. HMN supported the formation of tooth roots and periodontal tissues in developing tooth germs. In tooth germ organ culture, HMN promoted the elongation of Hertwig's epithelial root sheath (HERS) and stimulated cell proliferation in HERS and dental follicle-derived tissues, including dental papillae and dental follicles. HMN stimulated cell proliferation and cell movement of HERS-derived cells without mesenchymal cellsin vitroand directly induced the phosphorylation of SMAD1/5/8 protein in HERS-derived cells. Our results indicated that HMN was the first natural small compound to stimulate postnatal development of tooth germs.