The Architecture of the Cytoplasmic Region of Type III Secretion Systems.
The Architecture of the Cytoplasmic Region of Type III Secretion Systems.
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DOI：
10.1038/srep33341
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发表时间：
2016-09-30
影响因子：
4.6
通讯作者：
Blocker AJ
中科院分区：
文献类型：
--
作者：
Makino F;Shen D;Kajimura N;Kawamoto A;Pissaridou P;Oswin H;Pain M;Murillo I;Namba K;Blocker AJ
III型分泌系统（T3 SSs）是许多革兰氏阴性细菌病原体的毒力的基本装置。它们介导将细菌的毒力蛋白质效应物注射到真核宿主细胞中，以在感染期间操纵它们。毒力相关T3 SSs（vT 3SSs）与细菌鞭毛蛋白输出装置（fT 3SSs）在进化上相关，fT 3SSs对于鞭毛组装和细胞运动是必需的。fT 3SS和vT 3SS的外部和跨膜部分的结构越来越明确。然而，它们的细胞质和内膜输出装置的排列却不太清楚。在这里，我们比较的vT 3SS的福氏志贺菌和沙门氏菌血清型鼠伤寒沙门氏菌的vT 3SS和fT 3SS在~5和~4 nm分辨率的细胞质区域的结构，使用电子冷冻断层扫描和subtomogram平均。我们发现vT 3SSs的胞质区域显示保守的六重对称特征，包括豆荚，接头和ATP酶复合物，而fT 3SSs可能只显示六重对称的ATP酶区域。我们还确定了vT 3SSs和fT 3SSs之间的其他形态学差异，例如它们的内膜连接输出平台，C环/豆荚和ATP酶复合物的相对配置。最后，使用分类，我们发现，这两种类型的装置可以松散的细胞质区域的元素，因此可能是动态的。
Type III secretion systems (T3SSs) are essential devices in the virulence of many Gram-negative bacterial pathogens. They mediate injection of protein effectors of virulence from bacteria into eukaryotic host cells to manipulate them during infection. T3SSs involved in virulence (vT3SSs) are evolutionarily related to bacterial flagellar protein export apparatuses (fT3SSs), which are essential for flagellar assembly and cell motility. The structure of the external and transmembrane parts of both fT3SS and vT3SS is increasingly well-defined. However, the arrangement of their cytoplasmic and inner membrane export apparatuses is much less clear. Here we compare the architecture of the cytoplasmic regions of the vT3SSs of Shigella flexneri and the vT3SS and fT3SS of Salmonella enterica serovar Typhimurium at ~5 and ~4 nm resolution using electron cryotomography and subtomogram averaging. We show that the cytoplasmic regions of vT3SSs display conserved six-fold symmetric features including pods, linkers and an ATPase complex, while fT3SSs probably only display six-fold symmetry in their ATPase region. We also identify other morphological differences between vT3SSs and fT3SSs, such as relative disposition of their inner membrane-attached export platform, C-ring/pods and ATPase complex. Finally, using classification, we find that both types of apparatuses can loose elements of their cytoplasmic region, which may therefore be dynamic.
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影响因子：
3.1
作者：
Bahrani, FK;Sansonetti, PJ;Parsot, C
通讯作者：
Parsot, C
影响因子：
3.2
作者：
Ibuki, Tatsuya;Uchida, Yumiko;Minamino, Tohru
通讯作者：
Minamino, Tohru
影响因子：
4.6
作者：
Bai F;Morimoto YV;Yoshimura SD;Hara N;Kami-Ike N;Namba K;Minamino T
通讯作者：
Minamino T
DOI：
10.1073/pnas.1411610112
发表时间：
2015-01-27
影响因子：
11.1
作者：
Hu, Bo;Morado, Dustin R.;Liu, Jun
通讯作者：
Liu, Jun
影响因子：
2.2
作者：
Fernandez, J. J.;Li, S.;Crowther, R. A.
通讯作者：
Crowther, R. A.