Quantifying the effects of altering ambient humidity on ionic composition of vocal fold surface fluid.
Quantifying the effects of altering ambient humidity on ionic composition of vocal fold surface fluid.
复制标题
量化改变环境湿度对声带表面液体离子组成的影响。
DOI：
10.1002/lary.23924
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Rosen,ClarkA
中科院分区：
文献类型：
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作者：
Sivasankar,MPreeti;Carroll,ThomasL;Kosinski,AaronM;Rosen,ClarkA
目的/假设声带表面液（VFSF）在上皮细胞的水合和防御中起重要作用。本研究的目的是量化改变吸入空气的湿度后VFSF的离子组成的变化。我们测试的假设，即低湿度暴露会增加VFSF钠（Na+）和氯离子（Cl−）的浓度，但高湿度暴露会降低VFSF Na+和Cl−离子的浓度相比，低湿度challense.Study DesignProspective设计。在基线和暴露于低湿度和高湿度环境后从每个受试者收集VFSF。使用电感耦合等离子体质谱法评估VFSF Na+浓度。VFSF Cl−浓度用间接电位法测量。所有的分析都是由人员盲目的假设tested.ResultsThe低湿度环境中增加的Na+浓度在大多数的主题。Cl−浓度变化的数据是可变的。总体而言，数据未达到统计学显著性（P> 0.05）。主观印象表明，VFSF收集更困难，在低湿度相比，高湿度和基线conditions.ConclusionsThis研究是第一次尝试测量的离子浓度的VFSF。从目前的研究结果有重要的意义，未来的规划研究量化污染物和咽喉反流的影响VFSF组成，上皮水合作用，和声带防御。
Objectives/HypothesisVocal fold surface fluid (VFSF) is important in hydration and defense of underlying epithelial cells. The objective of this study was to quantify changes in the ionic composition of VFSF after altering the humidity of inhaled air. We tested the hypothesis that low humidity exposure would increase the concentration of VFSF sodium (Na+) and chloride (Cl−) ions but that high humidity exposure would decrease the concentration of VFSF Na+and Cl−ions as compared to the low humidity challenge.Study DesignProspective design.MethodsEighteen healthy adults participated in this study. VFSF was collected from each subject at baseline and following exposure to low humidity and high humidity environments. VFSF Na+concentration was assessed using inductively coupled plasma mass spectrometry. VFSF Cl−concentration was measured with indirect potentiometry. All analyses were completed by personnel blinded to the hypothesis being tested.ResultsThe low humidity environment increased Na+concentration in the majority of the subjects. Data for changes in Cl−concentrations were variable. Overall the data did not reach statistical significance (P> .05). Subjective impressions suggested that VFSF collection was more difficult in low humidity as compared to the high humidity and baseline conditions.ConclusionsThis study is the first attempt to measure the ionic concentration of VFSF. The results from the current study have important implications for future programmatic research quantifying the effects of pollutants and laryngopharyngeal reflux on VFSF composition, epithelial hydration, and vocal fold defense.