Identification of a Novel Glycoside, Leptosin, as a Chemical Marker of Manuka Honey
Identification of a Novel Glycoside, Leptosin, as a Chemical Marker of Manuka Honey
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DOI：
10.1021/jf300068w
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发表时间：
2012-04-04
影响因子：
6.1
通讯作者：
Kikuzaki, Hiroe
中科院分区：
文献类型：
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作者：
Kato, Yoji;Umeda, Natsuki;Kikuzaki, Hiroe
作为初步研究，我们发现来自新西兰麦卢卡（Leptospermum scoparium）的蜂蜜抑制髓过氧化物酶（MPO）活性。在本研究中，我们利用色谱技术从麦卢卡蜂蜜中分离出两种抑制mpo的活性化合物。一种是丁香酸甲酯（MSYR），另一种是丁香酸甲酯4- o - β - d -gentiobiose （MSYR）的一种新型糖苷，以细尾草属植物命名为“lepptosin”。糖苷的含量为0.2 ~ 1.2 μ mol/g蜂蜜。瘦素仅在大洋洲地区的蜂蜜中被发现，并且在麦卢卡蜂蜜中大量存在，包括来自澳大利亚Leptospermum polygalifolium的果冻灌木蜂蜜。因此，瘦素可能是麦卢卡蜂蜜很好的化学标记物。有趣的是，麦卢卡蜂蜜中瘦素的浓度与独特麦卢卡因子（UMF）值呈正相关，UMF值表示为其杀菌活性的苯酚当量。
As a preliminary study, we have found that honey from manuka (Leptospermum scoparium) in New Zealand inhibits myeloperoxidase (MPO) activity. In this study, using a chromatographic technique, we isolated two active compounds for MPO-inhibition from manuka honey. One is methyl syringate (MSYR), and the other was identified as a novel glycoside of MSYR, methyl syringate 4-O-beta-D-gentiobiose, which has been named "leptosin" after the genus Leptospermum. The amount of the glycoside ranged from 0.2 to 1.2 mu mol/g honey. Leptosin was only found in honeys from the Oceania region, and abundantly in manuka honey including jelly bush honey from Leptospermum polygalifolium in Australia. Therefore, leptosin may be a good chemical marker for manuka honey. Interestingly, the concentration of leptosin in manuka honey was positively correlated with the unique manuka factor (UMF) value, which is expressed as phenol equivalents of its bactericidal activity.