FRET-based Molecular Tension Microscopy
FRET-based Molecular Tension Microscopy
复制标题
DOI：
10.1016/j.ymeth.2015.07.010
复制
发表时间：
2016-02-01
期刊：
影响因子：
4.8
通讯作者：
Borghi, Nicolas
中科院分区：
文献类型：
--
作者：
Gayrard, Charlene;Borghi, Nicolas
细胞产生并经历机械力，这些机械力可以在各种生理或病理情况下塑造组织并调节信号传导途径。力如何在分子水平上传播和传播信号还知之甚少。基于FRET的分子张力显微镜的出现现在允许在分子尺度上实现具有分子特异性的原位机械力测量，从而更好地了解细胞和组织的机械结构以及机械转导途径。在这篇综述中，我们将首先揭示基于FRET的MTM的基本原理及其各种体现。我们将描述测量FRET的不同方法，它们的优点和缺点。然后，在整个感兴趣的蛋白质，细胞和生物体的范围内，它已被应用，我们将审查开发的测试来验证该方法，分子张力是如何与细胞功能，并得出结论与可能的发展和分支。(C)2015作者爱思唯尔公司出版
Cells generate and experience mechanical forces that may shape tissues and regulate signaling pathways in a variety of physiological or pathological situations. How forces propagate and transduce signals at the molecular level is poorly understood. The advent of FRET-based Molecular Tension Microscopy now allows to achieve mechanical force measurements at a molecular scale with molecular specificity in situ, and thereby better understand the mechanical architecture of cells and tissues, and mechanotransduction pathways. In this review, we will first expose the basic principles of FRET-based MTM and its various incarnations. We will describe different ways of measuring FRET, their advantages and drawbacks. Then, throughout the range of proteins of interest, cells and organisms to which it has been applied, we will review the tests developed to validate the approach, how molecular tension was related to cell functions, and conclude with possible developments and offshoots. (C) 2015 The Authors. Published by Elsevier Inc.