Inhibitory effects of linalool, an essential oil component of Lavender, on nociceptive TRPA1 and voltage-gated Ca2+ channels in mouse sensory neurons
Inhibitory effects of linalool, an essential oil component of Lavender, on nociceptive TRPA1 and voltage-gated Ca2+ channels in mouse sensory neurons
复制标题
薰衣草精油成分芳樟醇对小鼠感觉神经元伤害感受 TRPA1 和电压门控 Ca2 通道的抑制作用
DOI：
10.1016/j.bbrep.2023.101468
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发表时间：
2023
影响因子：
2.7
通讯作者：
Ohta T
中科院分区：
文献类型：
--
作者：
Hashimoto M;Takahashi K;Ohta T
芳樟醇是熏衣草的一种精油成分，通常用于香水。已知芳樟醇具有抗焦虑、镇静和镇痛作用。然而，其镇痛作用的机制尚未完全阐明。由外周神经元上的伤害感受器的激活引起的疼痛信号被传递到中枢神经系统。在本研究中，我们研究了芳樟醇对瞬时受体电位（TRP）通道和电压门控通道的影响，这两个通道对体感神经元中通过伤害性感受器的疼痛信号传导都很重要。为了检测通道活性，使用Ca 2+成像系统测量细胞内Ca 2+浓度（[Ca 2 +]i），并使用全细胞膜片钳技术记录膜电流。在小鼠感觉神经元中，芳樟醇在不引起[Ca ~（2+）] i升高的浓度下不影响对辣椒素和TRPV 1激动剂酸的[Ca ~（2+）] i反应，但抑制异硫氰酸烯丙酯（AITC）和TRPA 1激动剂香芹酚诱导的[Ca ~（2+）] i反应。在异源表达TRPA 1的细胞中观察到芳樟醇的类似抑制作用。芳樟醇减弱KCl和电压门控性Ca ~（2+）电流引起的[Ca ~（2+）] i增加，但仅轻微抑制电压门控性Na ~+电流。芳樟醇可抑制TRPA 1介导的伤害性行为，提示芳樟醇通过抑制TRPA 1和电压门控性Ca ~（2+）通道发挥镇痛作用。
Linalool, an essential oil component of lavender is commonly used in fragrances. It is known that linalool has anxiolytic, sedative, and analgesic actions. However, the mechanism of its analgesic action has not yet been fully clarified. Pain signals elicited by the activation of nociceptors on peripheral neurons are transmitted to the central nervous system. In the present study, we investigated the effects of linalool on transient receptor potential (TRP) channels and voltage-gated channels, both of which are important for pain signaling via nociceptors in somatosensory neurons. For detection of channel activity, the intracellular Ca2+concentration ([Ca2+]i) was measured using a Ca2+-imaging system, and membrane currents were recorded using the whole-cell patch-clamp technique. Analgesic actions were also examined in vivo.In mouse sensory neurons linalool at concentrations that did not induce [Ca2+]iincreases did not affect [Ca2+]iresponses to capsaicin and acids, TRPV1 agonists, but suppressed those induced by allyl isothiocyanate (AITC) and carvacrol, TRPA1 agonists. Similar inhibitory effects of linalool were observed in cells that heterologously expressed TRPA1. Linalool attenuated the [Ca2+]iincreases induced by KCl and voltage-gated Ca2+currents but only slightly suppressed voltage-gated Na+currents in mouse sensory neurons. Linalool diminished TRPA1-mediated nociceptive behaviors.The present data suggest that linalool exerts an analgesic action via the suppression of nociceptive TRPA1 and voltage-gated Ca2+channels.