Biochemical analysis of urethral collagen content after tubularized incised plate urethroplasty: an experimental study in rabbits
Biochemical analysis of urethral collagen content after tubularized incised plate urethroplasty: an experimental study in rabbits
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DOI：
10.1007/s00240-004-0407-9
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发表时间：
2004-02
影响因子：
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通讯作者：
F. Taneli;C. Ulman;A. Genç;O. Yilmaz;C. Taneli
中科院分区：
文献类型：
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作者：
F. Taneli;C. Ulman;A. Genç;O. Yilmaz;C. Taneli
本研究的目的是在切开的尿道板中组织羟脯氨酸水平的生物化学分析，以显示在尿道下裂兔模型中的Stimulgrass手术后的总胶原含量。本研究包括21只雄性新西兰兔（2.2-2.4 kg）。将动物随机分为3组，每组7只，如下：第1组，完全切除腹侧尿道并形成尿道下裂模型。在远端尿道背侧做全层切口，放置饲管作为导尿管，腹侧缝合两个尿道翼。第2组，将虹膜刀插入尿道，模仿尿道切开器切开腹壁。第3组为正常对照组，测定基底组织羟脯氨酸含量。与第1组和第2组的尿道组织背侧部分相比，在尿道组织腹侧部分观察到羟脯氨酸水平略有增加;然而，这些差异不显著。在兔模型中，在S草手术后，未观察到尿道背侧和腹侧部分的羟脯氨酸水平或它们与对照组之间的显著差异。需要进一步的研究，以确定尿道愈合的机制，通过正常的再上皮化，没有多余的胶原蛋白沉积后切开尿道板尿道成形术。
The aim of the present study was the biochemical analysis of tissue hydroxyproline levels in incised urethral plates in order to show the total collagen content after the Snodgrass operation in the hypospadiac rabbit model. The study comprised 21 male New Zealand rabbits, (2.2–2.4 kg). The animals were randomly allocated to three groups each containing seven rabbits as follows: group 1, the ventral urethra was completely excised and a model of hypospadias formed. A full-thickness incision was made on the distal dorsal urethra, a feeding tube was placed as an urethral catheter and both urethral wings were sutured ventrally.Group 2, inserting an iris knife into the urethra, the ventral wall was incised mimicking an urethrotome. Group 3 consisted of normal control rabbits to determine the basal tissue hydroxyproline level. A slight increase in the hydroxyproline level was observed in the ventral part of the urethral tissue compared to the dorsal part in both groups 1 and 2; however, these differences were not significant. After the Snodgrass operation in the rabbit model, no significant differences were observed in the hydroxyproline levels of the dorsal and ventral parts of the urethra or between these and of the controls. Further studies are required in order to determine the mechanism underlying urethral healing through normal re-epithelization without excess collagen deposition after incised urethral plate urethroplasty.