Encapsulation of Lactoferrin for Sustained Release Using Particles from Gas-Saturated Solutions
Encapsulation of Lactoferrin for Sustained Release Using Particles from Gas-Saturated Solutions
复制标题
使用气体饱和溶液中的颗粒封装乳铁蛋白以实现持续释放
DOI：
10.3390/pr9010073
复制
发表时间：
2020
期刊：
影响因子：
3.5
通讯作者：
K. Mishima
中科院分区：
文献类型：
--
作者：
Kento Ono;Hiroki Sakai;Shin;T. Sharmin;T. Aida;K. Mishima
采用超临界二氧化碳（scCO 2）对乳铁蛋白（一种具有铁结合性的乳糖蛋白）进行了气体饱和溶液（PGSS）法颗粒包埋研究。采用天然肠溶聚合物虫胶作为PGSS法制备乳铁蛋白的包衣材料。通过施加不同的温度（20-50 °C）和压力（8-10 MPa）来优化条件，并评估颗粒的颗粒形状和尺寸、乳铁蛋白包封效率、傅里叶变换红外（FTIR）光谱以确认乳铁蛋白包封和在不同pH值下的体外溶出研究。制备的微球平均粒径为75.5 ± 7 μm，包封率为71 ± 2%。此外，在低pH（pH 1.2）下显示高稳定性和在较高pH（pH 7.4）下随时间持续释放（t2 h = 75%）的颗粒表明用于保护乳铁蛋白免于胃消化的有效包封方法。
The particles from gas saturated solutions (PGSS) process were performed to encapsulate lactofer-rin, an iron-binding milk glycoprotein, using supercritical carbon dioxide (scCO2). A natural en-teric polymer, shellac, was used as a coating material of lactoferrin carried out by the PGSS pro-cess. Conditions were optimized by applying different temperatures (20–50 °C) and pressures (8–10 MPa) and the particles were evaluated for particle shape and size, lactoferrin encapsulation ef-ficiency, Fourier transform infrared (FTIR) spectroscopy to confirm lactoferrin entrapment and in vitro dissolution studies at different pH values. Particles with an average diameter of 75.5 ± 7 μm were produced with encapsulation efficiency up to 71 ± 2%. Furthermore, particles that showed high stability in low pH (pH 1.2) and a sustained release over time (t2h = 75%) in higher pH (pH 7.4) suggested an effective encapsulation process for the protection of lactoferrin from gastric di-gestion.