On-chip high-pressure picoliter injector for pressure-driven flow through porous media.
On-chip high-pressure picoliter injector for pressure-driven flow through porous media.
复制标题
片上高压皮升注射器用于压力驱动流过多孔介质。
作者：
D. Reichmuth;T. Shepodd;B. Kirby
高压 (> 3 MPa) 片上注射器已开发用于包括 HPLC 在内的微芯片应用。机械注射器是利用湿法蚀刻二氧化硅微芯片内的氟化丙烯酸酯的原位光聚合来实现的。该进样器允许重复进样量小至 180 pL，持续时间 < 250 ms。该注射器稳定运行超过 60 天，注射次数超过 1000 次。该注射器在聚合物阀门中是独一无二的，因为它可以在高压下的水、乙腈和混合缓冲液中工作，而不会检测到泄漏。
A high-pressure (> 3 MPa) on-chip injector has been developed for microchip applications including HPLC. The mechanical injector is implemented using in situ photopolymerization of fluorinated acrylates inside wet-etched silica microchips. The injector allows reproducible injections as small as 180 pL with < 250 ms duration. The injector operated robustly over 60 days and over 1000 injections. The injector is unique among polymer-based valves as it functions in aqueous, acetonitrile, and mixed buffers at high pressures without detectable leakage.