Cytotoxic and cytokine-inducing properties of Candida glabrata in single and mixed oral infection models
Cytotoxic and cytokine-inducing properties of Candida glabrata in single and mixed oral infection models
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DOI：
10.1016/j.micpath.2006.12.003
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发表时间：
2007-04-01
影响因子：
3.8
通讯作者：
Dongari-Bagtzoglou, Anna
中科院分区：
文献类型：
--
作者：
Li, Lulu;Kashleva, Helena;Dongari-Bagtzoglou, Anna
口腔念珠菌病是一种常见的机会性感染，其中白色念珠菌是最常见的病原体，光滑念珠菌成为重要的病原体。光滑念珠菌经常与口腔病变中的白色念珠菌同时分离。尽管白色念珠菌已被证明可引发显着的细胞因子反应和细胞损伤，但光滑念珠菌尚未得到系统研究。本研究的目的是利用口腔粘膜体外模型，表征光滑念珠菌作为单一感染生物以及与白色念珠菌联合诱导促炎细胞因子反应和宿主损伤的能力。在单层口腔上皮细胞培养物中，与白色念珠菌相比，光滑念珠菌未能诱导显着的白细胞介素 1 α 和白细胞介素 8 细胞因子反应，并且显示出较低的细胞毒性。然而，光滑念珠菌引发的粒细胞巨噬细胞集落刺激因子反应明显高于白色念珠菌。在人体口腔粘膜和粘膜下层的三维 (3-D) 体外模型中，光滑念珠菌菌株表现出菌株依赖性组织损伤能力和粘膜区室的表面入侵。在 3-D 系统中，联合感染未能使宿主损伤程度超过单独感染白色念珠菌的水平。这些研究表明，光滑念珠菌以菌株特异性方式在人口腔上皮细胞中诱导细胞因子，但与白色念珠菌相比，其组织/细胞损伤能力较低。在测试的菌株中没有观察到光滑念珠菌和白色念珠菌在细胞因子诱导和宿主损伤方面的协同作用。 (c) 2007 Elsevier Ltd. 保留所有权利。
Oral candidiasis is a common opportunistic infection, with Candida albicans being the most prevalent etiologic agent and Candida glabrata emerging as an important pathogen. C glabrata is frequently co-isolated with C albicans from oral lesions. Although C albicans has been shown to trigger significant cytokine responses and cell damage, C glabrata has not been systematically studied yet. The purpose of this study was to characterize the ability of C glabrata to induce proinflammatory cytokine responses and host damage as a single infecting organism and in combination with C albicans, using in vitro models of the oral mucosa. In monolayer oral epithelial cell cultures, C glabrata failed to induce a significant interleukin-1 alpha and interleukin-8 cytokine response and showed lower cytotoxicity, compared to C albicans. However, C glabrata triggered a significantly higher granulocyte macrophage colony stimulating factor response than C albicans. C glabrata strains showed a strain-dependent tissue damaging ability and a superficial invasion of the mucosal compartment in a three-dimensional (3-D) in vitro model of the human oral mucosa and submucosa. In the 3-D system, co-infection failed to promote host damage beyond the levels of infection with C albicans alone. These studies indicate that C glabrata induces cytokines in human oral epithelium in a strain-specific manner, but its tissue/cell damaging ability, compared to C albicans, is low. Synergy between C glabrata and C albicans in cytokine induction and host damage was not observed with the strains tested. (c) 2007 Elsevier Ltd. All rights reserved.