Differential expression of Bordetella pertussis iron transport system genes during infection
Differential expression of Bordetella pertussis iron transport system genes during infection
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DOI：
10.1111/j.1365-2958.2008.06333.x
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发表时间：
2008-10-01
影响因子：
3.6
通讯作者：
Armstrong, Sandra K.
中科院分区：
文献类型：
--
作者：
Brickman, Timothy J.;Hanawa, Tomoko;Armstrong, Sandra K.
在小鼠呼吸道感染模型中，使用alcA-、bfeA-和bhuR-tnpR重组酶融合菌株检查了百日咳博德特氏菌alcaligin、肠杆菌素和血红素铁获取系统的时间表达模式。铁系统在体内差异表达，显示早期诱导的alcaligin和肠杆菌素铁载体系统，和延迟诱导血红素系统的方式与预测的变化在感染过程中的主机铁源的可用性。以前的混合感染竞争研究确定了碱性蛋白和血红素利用对B的重要性。百日咳在体内的生长和存活。在这项研究中，肠杆菌素系统的贡献，适合的B。在类似的竞争感染实验中使用野生型和肠杆菌素受体突变株证实了百日咳。作为与B的体内表达研究的相关性。百日咳铁系统的小鼠，血清从未感染和B。筛选百日咳感染的人供体与博德特氏菌铁抑制性细胞包膜蛋白的抗体反应性。百日咳患者血清识别多种铁抑制蛋白，包括已知的外膜受体alcaligin，肠杆菌素和血红素，支持假设B。百日咳是缺铁的，并且在自然感染期间对不同铁源的存在有反应。
Temporal expression patterns of the Bordetella pertussis alcaligin, enterobactin and haem iron acquisition systems were examined using alcA-, bfeA- and bhuR-tnpR recombinase fusion strains in a mouse respiratory infection model. The iron systems were differentially expressed in vivo, showing early induction of the alcaligin and enterobactin siderophore systems, and delayed induction of the haem system in a manner consistent with predicted changes in host iron source availability during infection. Previous mixed infection competition studies established the importance of alcaligin and haem utilization for B. pertussis in vivo growth and survival. In this study, the contribution of the enterobactin system to the fitness of B. pertussis was confirmed using wild-type and enterobactin receptor mutant strains in similar competition infection experiments. As a correlate to the in vivo expression studies of B. pertussis iron systems in mice, sera from uninfected and B. pertussis-infected human donors were screened for antibody reactivity with Bordetella iron-repressible cell envelope proteins. Pertussis patient sera recognized multiple iron-repressible proteins including the known outer membrane receptors for alcaligin, enterobactin and haem, supporting the hypothesis that B. pertussis is iron-starved and responds to the presence of diverse iron sources during natural infection.