A Novel 2-Acrylamide-6-Methoxybenzothiazole Fabricated Molecularly Imprinted Polymers for Direct Fluorescent Sensing of Alachlor
A Novel 2-Acrylamide-6-Methoxybenzothiazole Fabricated Molecularly Imprinted Polymers for Direct Fluorescent Sensing of Alachlor
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DOI：
10.1007/s10337-015-2998-4
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发表时间：
2015
期刊：
影响因子：
1.7
通讯作者：
Ming Li;F. Shen;Zhe Zhang;X. Ren
中科院分区：
文献类型：
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作者：
Ming Li;F. Shen;Zhe Zhang;X. Ren
本工作研制了一种荧光分子印迹聚合物传感器，用于痕量甲草胺的快速直接检测。以2-氨基-6-甲氧基苯并噻唑和丙烯酰氯为原料，通过一步反应合成了2-丙烯酰胺-6-甲氧基苯并噻唑，并将其作为荧光报告分子和功能单体，制备了甲草胺分子印迹聚合物。通过这种方式，结合事件产生了物理上可检测的荧光信号，因此可以直接对甲草胺进行定量，而无需任何进一步处理。结合实验表明，在1-150 μM范围内，聚合物的荧光强度随甲草胺浓度的增加而线性下降，检测限为0.5 μM。此外，该荧光传感器对甲草胺表现出明显的选择性超过其潜在的竞争分子S-异丙甲草胺，氰草津，氯氰菊酯。最后，该方法成功地应用于测定玉米种子样品中的痕量甲草胺，具有良好的回收率为95.58%至103.83%。所建立的方法对真实的样品中甲草胺残留量的测定具有很好的应用前景。
In this work, a fluorescent, molecularly imprinted polymer sensor was developed for the rapid and direct sensing of trace alachlor. 2-Acrylamide-6-methoxybenzothiazole, synthesized via a simple one-step reaction with 2-amino-6-methoxybenzothiazole and acryloyl chloride, was used as both fluorescent reporter and functional monomer to prepare an alachlor-imprinted polymer. In this way, binding events produced physically detectable fluorescence signals, and the alachlor could therefore be quantified directly without any further treatment. Binding experiments demonstrated that the fluorescence intensity of the resultant polymer decreased linearly with increases in the concentration of alachlor, in the range of 1–150 μM, and with a detection limit of 0.5 μM. In addition, the fluorescent sensor exhibited significant selectivity toward alachlor over its potentially competing molecules ofS-metolachlor, cyanazine, and cypermethrin. Finally, the proposed method was successfully applied for the determination of trace alachlor in corn seed samples, with excellent recoveries ranging from 95.58 to 103.83 %. The developed method shows great potential for the determination of residual alachlor in real samples.