The disc angiogenesis system.
The disc angiogenesis system.
复制标题
椎间盘血管生成系统。
DOI：
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
A. Allison
中科院分区：
文献类型：
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作者：
L. F. Fajardo;J. Kowalski;H. Kwan;S. Prionas;A. Allison
一个新的系统，在体内研究血管生成已经设计出来。它由一个聚乙烯醇泡沫的小圆盘组成，在两个平面上覆盖有Millipore过滤器，仅留下边缘作为细胞渗透到圆盘中的区域。血管生成激动剂或拮抗剂以及其他待研究的物质被放置在椎间盘的中心。这些物质的缓慢释放通过乙烯-乙酸乙烯酯共聚物的膜或通过使用琼脂糖来维持。通过远端皮肤切口将椎间盘皮下植入宿主动物体内。在小鼠中，对于含有血管生成刺激剂的椎间盘，检查椎间盘的最佳时间为植入后7至12天，对于不含刺激剂的椎间盘，检查椎间盘的最佳时间为植入后12至20天。在生长完成一段时间后，将椎间盘取出，固定并包埋在石蜡或甲基丙烯酸酯中。通过多种方法染色的内侧平面切片用于观察和测量血管和基质向椎间盘中的生长。无论是否受到刺激，这种生长都是向心的，并且可以通过简单的形态测量技术容易地定量。该系统已经用于小鼠，研究血管和成纤维细胞增殖成没有或含有血管生成刺激物（表皮生长因子，酸性成纤维细胞生长因子）的椎间盘。我们还利用椎间盘来证明高温对血管生长的抑制作用。这些应用的例子。椎间盘血管生成系统易于制备，价格低廉，耐受性良好，至少在小鼠中是如此。其简单性和可重复性使其适用于超出本文所述的广泛应用。
A new system for the study of angiogenesis in vivo has been devised. It consists of a small disc of polyvinyl alcohol foam, covered on both flat sides by Millipore filters, leaving only the edge as the area for cell penetration into the disc. Angiogenic agonists or antagonists, as well as other substances to be studied, are placed in the center of the disc. The slow release of these substances is maintained by a film of ethylene-vinyl acetate co-polymer, or by the use of agarose. The disc is implanted subcutaneously in the host animal through a distant skin incision. In mice, the optimal times for examination of the discs are 7 to 12 days after implantation for discs containing angiogenic stimulants and 12 to 20 days for those without stimulants. After a period of growth is completed, the disc is removed, fixed, and embedded in paraffin or methacrylate. Medial plane sections, stained by a variety of methods, are used to observe and measure the growth of vessels and stroma into the disc. Whether stimulated or not, this growth is centripetal and can be easily quantitated by simple morphometric technics. This system has already been used in mice, to study the proliferation of vessels and fibroblasts into discs devoid of, or containing angiogenic stimulants (epidermal growth factor, acidic fibroblastic growth factor). We have also utilized the discs to demonstrate the inhibition of vessel growth by hyperthermia. Examples of these applications are presented. The disc angiogenesis system is easy to prepare, inexpensive, and well tolerated, at least by mice. Its simplicity and reproducibility make it suitable for a wide range of applications beyond those described here.