CYANOPHAGE S-2L CONTAINS DNA WITH 2,6-DIAMINOPURINE SUBSTITUTED FOR ADENINE
CYANOPHAGE S-2L CONTAINS DNA WITH 2,6-DIAMINOPURINE SUBSTITUTED FOR ADENINE
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DOI：
10.1016/0042-6822(78)90104-6
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发表时间：
1978-01-01
期刊：
影响因子：
3.7
通讯作者：
VANYUSHIN, BF
中科院分区：
文献类型：
--
作者：
KHUDYAKOV, IY;KIRNOS, MD;VANYUSHIN, BF
S-2L噬菌体是一种裂解聚球藻属单细胞蓝藻的噬菌体，它有一个直径为56的多面体头部和一个长度为120 nm的灵活的非收缩尾部。在S-2L噬菌体中，2，6-二氨基嘌呤完全取代了腺嘌呤。这个碱基和相应的脱氧核糖核苷分别是从噬菌体DNA的酸性和酶解物中分离出来的，并通过光学和层析行为进行了鉴定。S-2L噬菌体DNA为线形双链，分子量在26×106~28×106之间。该DNA在氯化铯中的浮力密度为1.731克/厘米~3。2，6-二氨基嘌呤稳定了噬菌体DNA的二级结构，即后者的熔融温度(Tmis85.6°in 0.1×SSC)比相同碱基组成的通常含腺嘌呤的DNA高3.6°。
Cyanophage S-2L, which lyses some strains of unicellular cyanobacteria of the genusSynechococcus, has a polyhedral head 56 run in diameter and a flexible noncontractile tail 120 nm in length.In S-2L phage DNA, 2,6-diaminopurine is completely substituted for adenine. This base and the corresponding deoxyribonucleoside have been isolated from acid and enzymatic hydrolyzates of the phage DNA, respectively, and have been identified according to optical and chromatographic behavior. S-2L phage DNA is linear and double-stranded and has a molecular weight of 26 × 106to 28 × 106. The buoyant density of this DNA in CsCl is 1.731 g/cm3. 2,6-Diaminopurine stabilizes the secondary structure of phage DNA, i.e., the melting temperature of the latter (Tmis85.6° in 0.1×SSC) is 3.6° higher than that of the usual adenine-containing DNA of equivalent base composition.