Diploid somatic-hybrid plants regenerated from rice cultivars
Diploid somatic-hybrid plants regenerated from rice cultivars
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由水稻品种再生的二倍体体细胞杂交植物
DOI：
10.1007/bf00303510
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发表时间：
1988
影响因子：
5.4
通讯作者：
K. Hinata
中科院分区：
文献类型：
--
作者：
Kinya Toriyama;K. Hinata
水稻品种“山后”和“村崎代国”获得了体细胞杂交植株。由于“Murasakidaikoku”是双突变体，同时具有显性（紫色）和隐性（矮化）标记，因此体细胞杂交种很容易与其亲本区分开来。从花药愈伤组织中分离原生质体，电熔培养原生质体，不选择杂交细胞。在27株再生植株中，9株通过紫色和正常植株类型证明是杂交种，这些性状与同一亲本品种间有性f1杂交种的性状相同。体细胞杂种包括3个二倍体植株和6个三倍体植株。在自交后代中观察到亲本标记的分离。这些结果表明，水稻的二倍体杂种可以通过单倍体花药来源细胞的体细胞杂交而不是通过性杂交获得。
Somatic hybrid plants were obtained between rice cultivars ‘Yamahoushi’ and ‘Murasakidaikoku’. Since ‘Murasakidaikoku’ is a double mutant having both dominant (purple coloration) and recessive (dwarf) markers, the somatic hybrids can be easily distinguished from their parents. Protoplasts were isolated from anther-derived calli, and electrofused protoplasts were cultured without selection of hybrid cells. Out of 27 regenerated plants, 9 proved to be hybrids based on their purple coloration and normal plant type, traits which were identical to those of the sexual F1hybrid between the same parental cultivars. The somatic hybrids included three diploid and six triploid plants. Segregation of parental markers was observed in the selfed progenies. These results demonstrated that diploid hybrids of rice could be obtained through somatic hybridization between haploid anther-derived cells instead of by sexual hybridization.