Nitric oxide donor improves healing if applied on inflammatory and proliferative phase
Nitric oxide donor improves healing if applied on inflammatory and proliferative phase
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DOI：
10.1016/j.jss.2007.10.015
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发表时间：
2008-09-01
影响因子：
2.2
通讯作者：
Monte-Alto-Costa, Andrea
中科院分区：
文献类型：
--
作者：
Amadeu, Thais P.;Seabra, Amedea. B.;Monte-Alto-Costa, Andrea
背景。一氧化氮（NO）是创面修复过程中合成的重要分子。已有研究报道一氧化氮供体用于皮肤伤口修复，但其在不同愈合阶段的作用仍未阐明。本研究的目的是研究局部应用NO供体（s -亚硝基谷胱甘肽，GSNO）水凝胶对大鼠皮肤创面修复炎性(（inf）、增殖（(prol)）、炎性和增殖期（(inf+prol)）切除创面的影响。材料和方法。各组（对照组、GSNO（inf）、GSNO（prol）和GSNO(inf+prol)）在背表面切除创面，观察创面收缩和再上皮化情况。伤后14 d，创面及邻近皮肤用福尔马林固定，石蜡包埋。观察胶原纤维组织、肥大细胞、肌成纤维细胞和血管。损伤后5、7 d， GSNO（inf+prol）组创面收缩速度均快于对照组、GSNO（inf）组和GSNO（prol）组。局部应用GSNO可促进损伤后14 d的再上皮化，主要在GSNO（inf+prol）组。此外，与其他各组相比，GSNO（inf+prol）组胶原纤维成熟和组织组织改善，肉芽组织浅部和深部炎症细胞数量减少。NO在大鼠皮肤伤口修复的所有阶段都很重要，但如果应用于炎症和增殖阶段，伤口愈合的改善（加速伤口闭合，伤口再上皮化和肉芽组织组织）更令人印象深刻。(c) 2008爱思唯尔公司版权所有。
Background. Nitric oxide (NO) is an important molecule synthesized during wound repair. Studies have reported the use of NO donors on cutaneous wound repair, but their effects in different phases of healing are still not elucidated. The aim of this work was to investigate the effects of topical application of a NO donor (S-nitrosoglutathione, GSNO)-containing hydrogel on excisional wounds in the inflammatory ((inf), proliferative ((prol)), and inflammatory and proliferative phases ((inf+prol)) of rat cutaneous wound repair.Material and Methods. In each group (control, GSNO(inf), GSNO(prol), and GSNO(inf+prol)), excisional wounds on the dorsal surface were made and wound contraction and re-epithelialization were evaluated. Fourteen days after wounding, wounds and adjacent skin were formalin-fixed and paraffin-embedded. Collagen fibers organization, mast cells, myofibroblasts and vessels were evaluated.Results. Wound contraction of the GSNO(inf+prol) group was faster than control, GSNO(inf), and GSNO(prol) groups, 5 and 7 d after wounding. Topical application of GSNO accelerated re-epithelialization 14 d after wounding, mainly in GSNO(inf+prol) group. In addition, the GSNO(inf+prol) group showed improved collagen fibers maturation and tissue organization, and lower amount of inflammatory cells in the superficial and deep areas of the granulation tissue, compared with the other groups.Conclusions. NO is important in all phases of rat cutaneous wound repair, but if applied on inflammatory and proliferative phases, the improvement in wound healing (accelerating wound closure, wound re-epithelialization, and granulation tissue organization) is more impressive. (c) 2008 Elsevier Inc. All rights reserved.