Proinsulin, C-peptide, and insulin in normal subjects during an 8-h hyperglycemic clamp.
Proinsulin, C-peptide, and insulin in normal subjects during an 8-h hyperglycemic clamp.
复制标题
正常受试者在 8 小时高血糖钳夹期间的胰岛素原、C 肽和胰岛素。
DOI：
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发表时间：
1996
影响因子：
5.8
通讯作者：
Christian Binder
中科院分区：
文献类型：
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作者：
S. Hartling;M. Røder;B. Dinesen;Christian Binder
胰岛素原免疫反应物质(PIM)绝对或相对胰岛素浓度升高是非胰岛素依赖型糖尿病(NIDDM)患者的特征表现。本研究的目的是检测健康受试者8h或轻度高血糖(7-9 mmol/L)是否能诱导B细胞优先分泌PIM。对9例正常体重、无糖尿病的健康受试者的一级亲属进行连续8h葡萄糖输注，每10min测定一次血清胰岛素、C-肽和PIM浓度。在B细胞多肽逐渐升高后，达到一个稳定状态。4~8h胰岛素、C-肽和PIM浓度无进一步变化。在葡萄糖钳夹期间，空腹PIM/C肽和PIM/胰岛素比值分别从0.5%和2.3%增加到1.4%和7.6%。无论是对回归斜率的检验，还是对各个时间点的比较，2~8h的PIM/C-肽比值和3~8h的PIM/C-肽比值均无显著差异，这些结果不支持葡萄糖驱动本身增加会导致B细胞功能改变的假说。在健康受试者中，至少8小时的轻度高血糖不会逐渐改变B细胞功能。
Increased concentrations of proinsulin immunoreactive material (PIM) absolutely or relative to insulin is a characteristic finding in patients with non-insulin-dependent diabetes mellitus (NIDDM). The aim of this study was to test if 8 h or mild hyperglycemia (7-9 mmol/l) in healthy subjects could induce a preferential secretion of PIM from B cells. Serum concentrations of insulin, C-peptide and PIM were measured every 10 min during the 8 h of continuous glucose infusion in nine normal-weight healthy subjects without diabetes among their first-degree relatives. After a gradual rise in B-cell peptides, a steady state was reached. From 4 to 8 h no further difference in insulin, C-peptide or PIM concentration was found. Fasting PIM/C-peptide and PIM/insulin ratios of 0.5% and 2.3% increased during the glucose clamp to levels of 1.4% and 7.6%, respectively. Neither testing the regression slope nor comparing individual time points showed any significant difference for the PIM/C-peptide ratio from 2 to 8 h and for the PIM/insulin ratio from 3 to 8 h. These results do not support the hypothesis that an increased glucose drive per se results in an altered B-cell function with increasing PIM/C-peptide ratio. At least 8 h of mild hyperglycemia in healthy subjects does not progressively alter B-cell function.