Star Polymers with a Cationic Core Prepared by ATRP for Cellular Nucleic Acids Delivery
Star Polymers with a Cationic Core Prepared by ATRP for Cellular Nucleic Acids Delivery
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DOI：
10.1021/bm4003199
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发表时间：
2013-05-01
影响因子：
6.2
通讯作者：
Matyjaszewski, Krzysztof
中科院分区：
文献类型：
--
作者：
Cho, Hong Y.;Averick, Saadyah E.;Matyjaszewski, Krzysztof
通过原子转移自由基聚合（ATRP）制备具有阳离子核心的基于聚（乙二醇）（PEG）的星形聚合物，用于体外核酸（NA）递送。星形聚合物通过甲基丙烯酸2-(二甲氨基)乙酯(DMAEMA)和二甲基丙烯酸乙二醇酯(EGDMA)的ATRP合成。通过凝胶渗透色谱、zeta 电位和动态光散射对星形聚合物进行了表征。这些星形聚合物与质粒 DNA (pDNA) 或短干扰 RNA (siRNA) 双链体结合，形成用于细胞内递送的聚合体。这些带有 siRNA 或 pDNA 的复合物在 NA 递送中非常有效，特别是在星形聚合物重量或摩尔比相对较低的情况下，突出了 NA 释放在高效递送系统中的重要性。
Poly(ethylene glycol) (PEG)-based star polymers with a cationic core were prepared by atom transfer radical polymerization (ATRP) for in vitro nucleic acid (NA) delivery. The star polymers were synthesized by ATRP of 2-(dimethylamino)ethyl methacrylate (DMAEMA) and ethylene glycol dimethacrylate (EGDMA). Star polymers were characterized by gel permeation chromatography, zeta potential, and dynamic light scattering. These star polymers were combined with either plasmid DNA (pDNA) or short interfering RNA (siRNA) duplexes to form polyplexes for intracellular delivery. These polyplexes with either siRNA or pDNA were highly effective in NA delivery, particularly at relatively low star polymer weight or molar ratios, highlighting the importance of NA release in efficient delivery systems.