Arabidopsis Genes Encoding Mitochondrial Type II NAD(P)H Dehydrogenases Have Different Evolutionary Origin and Show Distinct Responses to Light1
Arabidopsis Genes Encoding Mitochondrial Type II NAD(P)H Dehydrogenases Have Different Evolutionary Origin and Show Distinct Responses to Light1
复制标题
编码 II 型线粒体 NAD(P)H 脱氢酶的拟南芥基因具有不同的进化起源并对光表现出不同的反应1
作者：
A. Michalecka;A. Svensson;F. Johansson;Stephanie C. Agius;U. Johanson;A. Brennicke;S. Binder;A. Rasmusson
In addition to proton-pumping complex I, plant mitochondria contain several type II NAD(P)H dehydrogenases in the electron transport chain.额外的酶允许从细胞质和基质 NAD(P)H 到泛醌的非能量守恒电子转移。我们研究了拟南芥中的 II 型 NAD(P)H 脱氢酶基因家族。 This model plant contains two and four genes closely related to potato (Solanum tuberosum) genes nda1 and ndb1, respectively.还存在一种新的同源物，称为 ndc1，与马铃薯 nda1 和 ndb1 具有较低但显着的相似性。所有基因都在植物的多个器官中表达。在 nda 基因中，nda1（而非 nda2）的表达依赖于光和昼夜节律调节，表明在光合作用相关的和其他呼吸 NADH 氧化中具有不同的作用。绿色荧光融合蛋白的表达和分级细胞的蛋白质印迹表明，所有三个基因家族的基因都编码专门针对线粒体的蛋白质。系统发育分析表明 ndc1 与蓝藻 II 型 NADH 脱氢酶基因相关，表明该基因通过叶绿体祖细胞进入真核细胞。然后 ndc1 应该被转移到细胞核并获得线粒体靶向蛋白质产物的信号。尽管 nda、ndb 和 ndc 基因来源不同，但它们具有相同的内含子位置。
In addition to proton-pumping complex I, plant mitochondria contain several type II NAD(P)H dehydrogenases in the electron transport chain. The extra enzymes allow the nonenergy-conserving electron transfer from cytoplasmic and matrix NAD(P)H to ubiquinone. We have investigated the type II NAD(P)H dehydrogenase gene families in Arabidopsis. This model plant contains two and four genes closely related to potato (Solanum tuberosum) genes nda1 and ndb1, respectively. A novel homolog, termed ndc1, with a lower but significant similarity to potato nda1 and ndb1, is also present. All genes are expressed in several organs of the plant. Among the nda genes, expression of nda1, but not nda2, is dependent on light and circadian regulation, suggesting separate roles in photosynthesis-associated and other respiratory NADH oxidation. Genes from all three gene families encode proteins exclusively targeted to mitochondria, as revealed by expression of green fluorescent fusion proteins and by western blotting of fractionated cells. Phylogenetic analysis indicates that ndc1 affiliates with cyanobacterial type II NADH dehydrogenase genes, suggesting that this gene entered the eukaryotic cell via the chloroplast progenitor. The ndc1 should then have been transferred to the nucleus and acquired a signal for mitochondrial targeting of the protein product. Although they are of different origin, the nda, ndb, and ndc genes carry an identical intron position.
DOI：
10.1073/pnas.97.13.6960
发表时间：
2000-06-20
影响因子：
11.1
作者：
Palmer, JD;Adams, KL;Song, KM
通讯作者：
Song, KM