Matrigel Plug Assay for In Vivo Evaluation of Angiogenesis
Matrigel Plug Assay for In Vivo Evaluation of Angiogenesis
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DOI：
10.1007/978-1-4939-9133-4_18
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发表时间：
2019-01-01
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影响因子：
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通讯作者：
Papadimitriou, Evangelia
中科院分区：
文献类型：
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作者：
Kastana, Pinelopi;Zahra, Fatema Tuz;Papadimitriou, Evangelia
Matrigel 是从 C57BL/6 小鼠的 Engelbreth-Holm-Swarm (EHS) 小鼠肉瘤中提取的，这是一种富含细胞外基质 (ECM) 蛋白的肿瘤。它主要由层粘连蛋白（约 60%）、IV 型胶原蛋白（约 30%）和 nidogen-1/entactin（约 8%）组成，同时还含有硫酸乙酰肝素蛋白聚糖，如基底膜聚糖、其他 ECM 蛋白以及与 ECM 结合的生长因子。基质胶模拟生理细胞基质，是研究体外和体内血管生成最常用的基质基质。在这里，我们描述了体内基质胶塞测定以及如何将其用于血管生成的定性和定量评估。
Matrigel is extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma in C57BL/6 mice, a tumor rich in extracellular matrix (ECM) proteins. It consists mainly of laminin (approximately 60%), collagen IV (approximately 30%), and nidogen-1/entactin (approximately 8%), while it also contains heparan sulfate proteoglycans, such as perlecan, other ECM proteins, as well as growth factors bound to the ECM. Matrigel mimics the physiological cell matrix and is the most commonly used matrix substrate to study in vitro and in vivo angiogenesis. Here, we describe the in vivo Matrigel plug assay and how it can be used for both qualitative and quantitative assessment of angiogenesis.