Carotenoids of an Antarctic psychrotolerant bacterium, Sphingobacterium antarcticus, and a mesophilic bacterium, Sphingobacterium multivorum
Carotenoids of an Antarctic psychrotolerant bacterium, Sphingobacterium antarcticus, and a mesophilic bacterium, Sphingobacterium multivorum
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DOI：
10.1007/s002030000163
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发表时间：
2000-05-01
影响因子：
2.8
通讯作者：
Shivaji, S
中科院分区：
文献类型：
--
作者：
Jagannadham, MV;Chattopadhyay, MK;Shivaji, S
南极耐冷细菌Sphingobacterium anastriticus和嗜温细菌Syhingobacterium multivorum的主要类胡萝卜素色素被鉴定为玉米黄质、β-隐黄质和β-胡萝卜素。分析基于紫外-可见光谱、质谱和反相HPLC。完整细菌细胞的光声光谱显示，S。anaiticus和S. multiorum与细胞膜相连。在体外研究与合成膜的磷脂酰胆碱表明，主要的色素是绑定到膜，并降低其流动性。在5 ℃下生长的细胞中极性色素的相对量高于在25 ℃下生长的细胞。在嗜温菌株中，极性类胡萝卜素的合成量小于耐冷菌株。
The major carotenoid pigments of an Antarctic psychrotolerant bacterium, Sphingobacterium antarcticus, and a mesophilic bacterium, Syhingobacterium multivorum, were identified as zeaxanthin, beta-cryptoxanthin, and beta-carotene. Analysis was based on ultraviolet-visible spectroscopy, mass spectroscopy, and reversed-phase HPLC. Photoacoustic spectroscopy of intact bacterial cells revealed that the bulk of the pigments in S. antarcticus and S. multivorum was associated with the cell membrane. In vitro studies with synthetic membranes of phosphatidylcholine demonstrated that the major pigment was bound to the membranes and decreased their fluidity. The relative amounts of polar pigments were higher in cells grown at 5 degrees C than in cells grown at 25 degrees C. In the mesophilic strain, the synthesis of polar carotenoids was quantitatively less than that of the psychrotolerant strain.