Blebbing dynamics during endothelial cell spreading
Blebbing dynamics during endothelial cell spreading
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DOI：
10.1016/j.ejcb.2010.09.013
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发表时间：
2011-01-01
影响因子：
6.6
通讯作者：
Aranda-Espinoza, Helim
中科院分区：
文献类型：
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作者：
Norman, Leann;Sengupta, Kheya;Aranda-Espinoza, Helim
细胞扩散是许多生理现象的重要组成部分，包括癌症转移、胚胎发育和有丝分裂。我们先前已经说明，细胞泡在细胞-底物突然分离后出现，并在调节膜张力方面发挥关键作用：然而，在扩散过程中，细胞泡-底物相互作用的动力学仍不清楚。在这里，我们使用化学和渗透修饰来诱导或抑制滤过泡的形成，来探讨滤过泡在内皮细胞扩散过程中的作用。我们使用表面敏感的显微技术跟踪细胞-基质动力学以及单个气泡。起泡细胞(对照和化学诱导)在快速生长之前表现出较长的滞后时间。有趣的是，与无气泡的细胞相比，气泡细胞的气泡出现得更晚，而且在所有情况下，气泡都发出快速扩散的信号。我们的结果表明，细胞泡在细胞扩散的早期阶段起着关键作用，首先是通过控制细胞的初始黏附，然后是通过动态地抑制细胞的扩散，直到出现薄层并随后快速扩散。(C)2010年爱思唯尔股份有限公司。版权所有。
Cell spreading is a critical component of numerous physiological phenomena including cancer metastasis, embryonic development, and mitosis. We have previously illustrated that cellular blebs appear after abrupt cell-substrate detachment and play a critical role in regulating membrane tension: however, the dynamics of bleb-substrate interactions during spreading remains unclear. Here we explore the role of blebs during endothelial cell spreading using chemical and osmotic modifications to either induce or inhibit bleb formation. We track cell-substrate dynamics as well as individual blebs using surface sensitive microscopic techniques. Blebbing cells (both control and chemically induced) exhibit increased lag times prior to fast growth. Interestingly, lamellae appear later for blebbing compared to non-blebbing cells, and in all cases, lamellae signal the start of fast spreading. Our results indicate that cellular blebs play a key role in the early stage of cell spreading, first by controling the initial cell adhesion and then by presenting a dynamic inhibition of cell spreading until a lamella appears and fast spreading ensues. (C) 2010 Elsevier GmbH. All rights reserved.