Role of IL-10 in the abnormalities of early cell activation events of lymphocytes from patients with systemic lupus erythematosus
Role of IL-10 in the abnormalities of early cell activation events of lymphocytes from patients with systemic lupus erythematosus
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DOI：
10.1006/jaut.1998.0228
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发表时间：
1998-10-01
影响因子：
12.8
通讯作者：
Alaron-Segovia, D
中科院分区：
文献类型：
--
作者：
Gonzalez-Amaro, R;Portales-Perez, D;Alaron-Segovia, D
Lymphocytes from patients with systemic lupus erythematosus (SLE) exhibit abnormalities in early cell activation events as well as increased production of IL-10. We explored the possible role of IL-10 on defective cell activation events of SLE lymphocytes and first studied the in vitro effect of IL-10 on peripheral blood mononuclear cells(PBMNC) from healthy subjects. After 5 days of culture in the presence of exogenous IL-10, these cells demonstrated abnormal expression of CD69 as well as high intracellular pH and defective activation of the Na+/H+ anti-porter by PMA. We then investigated the effect of IL-10 blockade on PBMNC from SLE patients. SLE PBMNC cultured for 5 days with a blocking anti-IL-10 monoclonal antibody (mAb) partially corrected abnormalities in CD69 expression and intracellular pH; however, in 1/5 patients studied, no significant positive effect was observed. The effect of the anti-IL-10 mAb was apparently not related to protection against activation-induced cell death. We conclude that IL-10 in normal PBMNC induce some of the defects in early cell activation events seen in SLE lymphocytes. Accordingly, the blockade of IL-10 partially corrects these abnormalities in SLE cells. Our data further support the importance of IL-10 in immune dysfunction seen in SLE. (C) 1998 Academic Press
