Antibodies Directed Against Integration Host Factor Mediate Biofilm Clearance From Nasopore
Antibodies Directed Against Integration Host Factor Mediate Biofilm Clearance From Nasopore
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DOI：
10.1002/lary.24183
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发表时间：
2013-11-01
期刊：
影响因子：
2.6
通讯作者：
Das, Subinoy
中科院分区：
文献类型：
--
作者：
Brandstetter, Kathleyn A.;Jurcisek, Joseph A.;Das, Subinoy
目的/假设鼻内可吸收填充物，如Nasopore，常用于鼻窦手术，尽管缺乏证据证明其临床益处。我们的理论是，Nasopore支持细菌生长和生物膜的形成。细菌核酸结合蛋白DNABII家族稳定生物膜的胞外聚合物质，从而保护细菌免受宿主防御和传统抗生素的侵害。我们检验了一种假设，即使用抗ihf抗体与抗生素联合使用将促进Nasopore的生物膜根除。研究设计体外实验。方法在鼻孔上培养非分型流感嗜血杆菌生物膜。24小时孵育后，将生物膜单独与培养基、兔血清、兔抗ihf血清、阿莫西林/克拉维酸或抗ihf血清+阿莫西林/克拉维酸孵育16小时。对共聚焦显微镜获得的生物膜图像进行计算机统计（COMSTAT）分析。结果snthi易在鼻孔上形成生物膜。与单独在无菌培养基中孵育(3.46（2)/(3)）相比，单独使用阿莫西林/克拉维酸处理可使生物量增加92%，达到6.63(2)/(3)。与用兔血清孵育(5.53（2)/(3)）相比，单独使用抗ihf治疗可使生物量减少77%，降至1.29(2)/(3)。抗ihf +阿莫西林/克拉维酸使生物量降低88%至0.66 (2)/(3)(P
Objectives/HypothesisIntranasal resorbable packing, such as Nasopore, is commonly used during sinus surgery despite a paucity of evidence that demonstrates clinical benefit. We theorized that Nasopore supports bacterial growth and biofilm formation. The DNABII family of bacterial nucleic acid binding proteins stabilizes the extracellular polymeric substance of the biofilm, thus protecting bacteria from host defenses and traditional antibiotics. We tested the hypothesis that use of anti-IHF antibodies in conjunction with antibiotics would enhance biofilm eradication from Nasopore.Study DesignIn vitro experiments.MethodsNontypeable Haemophilus influenzae (NTHI) biofilms were grown on Nasopore. Following 24-hour incubation, biofilms were incubated for an additional 16 hours with either medium alone, naive rabbit serum, rabbit anti-IHF serum, amoxicillin/clavulanate, or anti-IHF serum+amoxicillin/clavulanate. Computer statistics (COMSTAT) analysis was performed on images of biofilms obtained via confocal microscopy.ResultsNTHI readily formed a biofilm on Nasopore. Treatment with amoxicillin/clavulanate alone mediated an increase in biomass by 92% to 6.63 (2)/(3) compared to incubation in sterile medium alone (3.46 (2)/(3)). Treatment with anti-IHF alone reduced the biomass by 77% to 1.29 (2)/(3) compared to incubation with naive rabbit serum (5.53 (2)/(3)). Anti-IHF+amoxicillin/clavulanate reduced biomass by 88% to 0.66 (2)/(3) (P