METHOD FOR DETECTION OF SPECIFIC RNAS IN AGAROSE GELS BY TRANSFER TO DIAZOBENZYLOXYMETHYL-PAPER AND HYBRIDIZATION WITH DNA PROBES
METHOD FOR DETECTION OF SPECIFIC RNAS IN AGAROSE GELS BY TRANSFER TO DIAZOBENZYLOXYMETHYL-PAPER AND HYBRIDIZATION WITH DNA PROBES
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DOI：
10.1073/pnas.74.12.5350
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发表时间：
1977-01-01
影响因子：
11.1
通讯作者：
STARK, GR
中科院分区：
文献类型：
--
作者：
ALWINE, JC;KEMP, DJ;STARK, GR
描述了一种将琼脂糖凝胶上分离的RNA条带转移到纸条上的技术。RNA与纸上的重氮苄氧基甲基共价偶联。在转移和适当处理纸以破坏剩余的重氮基团后，可以通过与32P标记的DNA探针杂交，然后进行放射自显影来检测特异性RNA条带。该程序允许以高灵敏度和低背景检测特异性[黑腹果蝇] RNA条带。
A technique is described for transferring electrophoretically separated bands of RNA from an agarose gel to paper strips. The RNA is coupled covalently to diazobenzyloxymethyl groups on the paper. After transfer and appropriate treatment of the paper to destroy remaining diazo groups, specific RNA bands can be detected by hybridization with 32P-labeled DNA probes followed by autoradiography. This procedure allows detection of specific [Drosophila melanogaster] RNA bands with high sensitivity and low background.