Copan Walk Away Specimen Processor (WASP) Automated System for Pathogen Detection in Female Reproductive Tract Specimens.
Copan Walk Away Specimen Processor (WASP) Automated System for Pathogen Detection in Female Reproductive Tract Specimens.
复制标题
用于女性生殖道样本病原体检测的 Copan 样本处理机 (WASP) 自动化系统
DOI：
10.3389/fcimb.2021.770367
复制
发表时间：
2021
影响因子：
5.7
通讯作者：
Ying C
中科院分区：
文献类型：
--
作者：
Gao J;Chen Q;Peng Y;Jiang N;Shi Y;Ying C
自动化越来越多地应用于临床实验室;然而，由于涉及复杂的程序，微生物检测和筛选的分析前处理仍然主要使用手动方法进行。为了促进临床微生物实验室的自动化，必须分别评估自动化系统对不同样本类型的性能。因此，本研究的目的是探讨科潘Walk Away Specimen Processor（WASP）自动分析前微生物处理系统在女性生殖道标本中病原体检测的潜在临床应用及其优化诊断程序的可行性。方法收集首次产检孕妇的生殖道标本，采用科潘WASP自动化标本处理系统接种于培养液中，并采用传统的人工接种方法进行培养。培养48 h后，观察菌落生长情况，比较自动组和手动组的细菌类型、菌落数和分离单菌落的效率。通过荧光定量聚合酶链反应（qPCR）和免疫色谱法进一步分析使用Copan-ESwab样本采集管从WASP系统采集的标本中是否存在沙眼衣原体（CT）、淋病奈瑟菌（NG）和解脲支原体（UU），以研究该方法优化女性生殖道这些常见病原体检测的可行性。结果与手工培养法相比，科潘WASP微生物自动化系统检出菌型和菌落数均减少（P <0.001），但单菌落检出率较高（P <0.001）。两种方法对妇产科常见致病菌的检出率比较，B族链球菌（GBS）（P=0.575）和念珠菌（P=0.917）差异无统计学意义。Copan-ESwab管中采集的标本可用于通过基于荧光的qPCR筛查GBS和CT，但不能用于免疫层析。然而，使用两种方法均未在任何样本中检出UU和NG;因此，需要进一步验证以确定科潘系统筛查这些病原体的可行性。结论科潘WASP微生物自动检测系统可优化女性生殖系统常见病原体的检测流程，降低相关成本。
Objective Automation is increasingly being applied in clinical laboratories; however, preanalytical processing for microbiology tests and screening is still largely performed using manual methods owing to the complex procedures involved. To promote automation of clinical microbiology laboratories, it is important to assess the performance of automated systems for different specimen types separately. Therefore, the aim of this study was to explore the potential clinical application of the Copan Walk Away Specimen Processor (WASP) automated preanalytical microbiology processing system in the detection of pathogens in female reproductive tract specimens and its feasibility in optimizing diagnostic procedures. Methods Female reproductive tract specimens collected from pregnant women at their first obstetric check-up were inoculated into culture media using the Copan WASP automated specimen processing system and were also cultured using a conventional manual inoculation method. After 48 h of culture, the growth of colonies was observed, and the types of bacteria, number of colonies, and efficiency in isolating single colonies were compared between the automated and manual groups. The specimens collected from the WASP system using the Copan-ESwab sample collection tubes were further analyzed for the presence of Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), and Ureaplasmaurealyticum (UU) via fluorescence quantitative polymerase chain reaction (qPCR) and an immunochromatographic assay to investigate the feasibility of this method in optimizing detection of these common pathogens of the female reproductive tract. Results Compared with the manual culture method, the Copan WASP microbiology automation system detected fewer bacterial types (P<0.001) and bacterial colonies (P<0.001) but had a higher detection rate of single colonies (P<0.001). There was no significant difference in the detection rates of common pathogens encountered in clinical obstetrics and gynecology, including group B Streptococcus (GBS) (P=0.575) and Candida (P=0.917), between the two methods. Specimens collected in the Copan-ESwab tubes could be used for screening of GBS and CT via fluorescence-based qPCR but not with immunochromatography. However, UU and NG were not detected in any sample with either method; thus, further validation is required to determine the feasibility of the Copan system for screening these pathogens. Conclusion The Copan WASP microbiology automation system could facilitate the optimization of diagnostic procedures for detecting common pathogens of the female reproductive system, thereby reducing associated costs.