Natural killer cell cytotoxic activity and c-Fos protein synthesis in rat hypothalamic cells after painful electric stimulation of the hind limbs and EHF irradiation of the skin.
Natural killer cell cytotoxic activity and c-Fos protein synthesis in rat hypothalamic cells after painful electric stimulation of the hind limbs and EHF irradiation of the skin.
复制标题
后肢疼痛电刺激和皮肤 EHF 照射后大鼠下丘脑细胞自然杀伤细胞的细胞毒活性和 c-Fos 蛋白合成。
DOI：
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发表时间：
2005
期刊：
影响因子：
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通讯作者：
E. Korneva
中科院分区：
文献类型：
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作者：
S. N. Shanin;E. Rybakina;N. Novikova;I. A. Kozinets;V. Rogers;E. Korneva
背景
目的是评估疼痛电刺激诱导的下丘脑结构中自然杀伤（NK）细胞活性和c-Fos阳性细胞数量的变化，并使用极高频（EHF）照射皮肤来调节这些过程。
材料/方法
实验在Wistar大鼠后肢疼痛电刺激结合EHF皮肤照射的条件下进行。脾NK细胞的细胞毒活性通过它们在体外裂解K-562肿瘤细胞的能力来评估。免疫过氧化物酶法检测c-Fos样蛋白。
结果
疼痛性电刺激与脾NK细胞毒性的显著降低和下丘脑某些结构中c-Fos阳性细胞计数的急剧增加相关，特别是在下丘脑前核（AHN）和下丘脑穹窿周围外侧区（LHA）。两个EHF暴露，一个之前和一个之后的电刺激，防止脾NK细胞活性的抑制，并导致c-Fos阳性细胞的数量减少，在下丘脑腹内侧核（VMH）和基底LHA。AHN和LHA中c-Fos阳性细胞数与NK细胞杀伤活性呈负相关。
结论
这些结果表明，大鼠后肢的痛苦的电刺激导致重组的中央机制，调节脾NK细胞的活性，导致其细胞毒性的降低，和EHF照射的皮肤防止这种重组，从而保护脾NK细胞的活性从这种应激源引起的损害。
BACKGROUND
The goal was to assess changes in natural killer (NK) cell activity and the number of c-Fos-positive cells in hypothalamic structures induced by painful electrical stimulation and to use extremely high-frequency (EHF) irradiation of the skin to modulate these processes.
MATERIAL/METHODS
Experiments were performed on Wistar rats subjected to painful electrical stimulation of the hind limbs combined with EHF irradiation of the skin. The cytotoxic activity of splenic NK cells was assessed by their ability to lyse K-562 tumor cells in vitro. c-Fos-like protein was detected by an immunoperoxidase technique.
RESULTS
Painful electric stimulation was associated with a significant decrease in splenic NK cytotoxicity and a dramatic increase in c-Fos-positive cell counts in some hypothalamic structures, particularly in the anterior hypothalamic nucleus (AHN) and the perifornical lateral hypothalamic area (LHA). Two EHF exposures, one before and one after electric stimulation, prevented the suppression of splenic NK cell activity and caused a decrease in the number of c-Fos-positive cells expressed in the ventromedial hypothalamic nucleus (VMH) and basal LHA. Negative correlation was shown between c-Fos-positive cell counts in the AHN and LHA and the cytotoxic activity of NK cells.
CONCLUSIONS
These results suggest that painful electric stimulation of the hind limbs of rats causes a reorganization of the central mechanisms that regulate splenic NK cell activity, resulting in a decrease in their cytotoxicity, and that EHF irradiation of the skin prevents this reorganization, thus protecting splenic NK cell activity from the impairment induced by this stressor.